PA5-31559

antibody from Invitrogen Antibodies

Targeting: UBR7 C14orf130

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31559

Provider

Invitrogen Antibodies

Product name

C14orf130 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: U87-MG. Predicted reactivity: Mouse (83%), Rat (83%), Rhesus Monkey (97%), Bovine (86%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Manumycin polyketides act as molecular glues between UBR7 and P53.

Isobe Y, Okumura M, McGregor LM, Brittain SM, Jones MD, Liang X, White R, Forrester W, McKenna JM, Tallarico JA, Schirle M, Maimone TJ, Nomura DK
Nature chemical biology 2020 Nov;16(11):1189-1198

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Insights into the ubiquitin-proteasome system of human embryonic stem cells.

Saez I, Koyuncu S, Gutierrez-Garcia R, Dieterich C, Vilchez D
Scientific reports 2018 Mar 6;8(1):4092

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The ubiquitin ligase UBR5 suppresses proteostasis collapse in pluripotent stem cells from Huntington's disease patients.

Koyuncu S, Saez I, Lee HJ, Gutierrez-Garcia R, Pokrzywa W, Fatima A, Hoppe T, Vilchez D
Nature communications 2018 Jul 23;9(1):2886

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using C14orf130 Polyclonal Antibody (Product # PA5-31559). Sample (30 µg of whole cell lysate). Lane A: U87-MG. 12% SDS PAGE. C14orf130 Polyclonal Antibody (Product # PA5-31559) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of UBR7 in paraformaldehyde-fixed MCF-7 cells using a UBR7 polyclonal antibody (Product # PA5-31559) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

C14orf130 Polyclonal Antibody detects UBR7 protein at nucleus on Huh-7 hepatocellular carcinoma xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Huh-7 hepatocellular carcinoma xenograft. C14orf130 Polyclonal Antibody (Product # PA5-31559) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Loss of UBR7, UBE3A and RNF181 does not affect the expression of pluripotency markers and neural differentiation. (a) Western blot analysis with antibodies to UBR7 and OCT4 comparing non-targeting shRNA (NT) with UBR7 knockdown (KD) H9 hESCs. We used two independent shRNAs to UBR7 (KD1 and KD2, respectively). ß-actin is the loading control. The images are representative of two independent experiments. All cropped blots were run under the same experimental conditions. The original blots are included in Supplementary Fig. 13. (b) Real-time PCR analysis of pluripotency (left panel) and germ-layer markers (right panel) in UBR7 KD H9 hESCs. Graphs (relative expression to NT shRNA) represent the mean s.e.m. of four independent experiments. (c) Western blot analysis after 10 days of neural induction of UBR7 KDH9 hESCs. ß-actin is the loading control. The images are representative of two independent experiments. (d) Immunocytochemistry after 10 days of neural differentiation. OCT4, PAX6, and DAPI staining were used as markers of pluripotency, neuroectodermal differentiation, and nuclei, respectively. Scale bar represents 20 µm. (e) Real-time PCR analysis of pluripotency (upper panel) and neuroectodermal markers (lower panel) in UBR7 KD H9 hESCs after 10 days of neural differentiation. Graphs (relative expression to NT) represent the mean s.e.m. of three independent experiments. (f,g) Western blots of UBE3A and OCT4 (f) and RNF181 and OCT4 (g) protein levels. ß-actin is the loadin|