Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-30957 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytoglobin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: IMR32, mouse heart, PC-12. Predicted reactivity: Human (99%), Mouse (94%), Rat (93%), Dog (94%), Sheep (96%), Rhesus Monkey (97%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Cytoglobin was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Cytoglobin Polyclonal Antibody (Product # PA5-30957) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cytoglobin Polyclonal Antibody (Product # PA5-30957). Sample (50 µg of whole cell lysate). Lane A: mouse heart. 12% SDS PAGE. Cytoglobin Polyclonal Antibody (Product # PA5-30957) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cytoglobin Polyclonal Antibody (Product # PA5-30957). Sample (30 µg of whole cell lysate). Lane A: IMR32. 12% SDS PAGE. Cytoglobin Polyclonal Antibody (Product # PA5-30957) diluted at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cytoglobin in methanol-fixed A431 cells using a Cytoglobin polyclonal antibody (Product # PA5-30957) at a 1:500 dilution.