H00004838-M05

antibody from Abnova Corporation

Targeting: NODAL

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004838-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004838-M05, RRID:AB_606669

Product name

NODAL monoclonal antibody (M05), clone 5A3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant NODAL.

Antigen sequence

RCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVP
STCCAPVKTKPLSMLYVDNGRVLLDHHKDMIVEEC
GC


Isotype

IgG

Antibody clone number

5A3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

NODAL monoclonal antibody (M05), clone 5A3 Western Blot analysis of NODAL expression in HeLa ( Cat # L013V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

NODAL monoclonal antibody (M05), clone 5A3. Western Blot analysis of NODAL expression in HepG2 ( Cat # L019V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

NODAL monoclonal antibody (M05), clone 5A3. Western Blot analysis of NODAL expression in PC-12 ( Cat # L012V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged NODAL is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol