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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- PA5-28486 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nodal Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Human ESC. Predicted reactivity: Mouse (91%), Rat (92%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nodal in Mouse tissue extract (50 µg). Samples was separated by 10% SDS-PAGE and the membrane was probed with Nodal Polyclonal antibody (Product # PA5-28486) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Nodal Polyclonal Antibody (Product # PA5-28486). Sample (20 µg of whole cell lysate). Lane A: Human ESC. 10% SDS PAGE. Nodal Polyclonal Antibody (Product # PA5-28486) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Nodal Polyclonal Antibody (Product # PA5-28486). Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Nodal Polyclonal Antibody (Product # PA5-28486) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA detection of NODAL using Nodal Polyclonal Antibody (Product # PA5-28486) for capture at a concentration of 5 µg/mL and a NODAL Polyclonal Antibody for detection at a concentration of 1.5 µg/mL.
