GTX102784

antibody from GeneTex

Targeting: TGFBR1 ACVRLK4, ALK-5, ALK5, ESS1, MSSE, TBR-i, TBRI

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX102784

Provider

GeneTex

Proper citation

GeneTex Cat#GTX102784, RRID:AB_10723107

Product name

TGF beta Receptor I antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat, Porcine

Host

Rabbit

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References

Submitted references

Triazole RGD antagonist reverts TGFβ1-induced endothelial-to-mesenchymal transition in endothelial precursor cells.

Bianchini F, Peppicelli S, Fabbrizzi P, Biagioni A, Mazzanti B, Menchi G, Calorini L, Pupi A, Trabocchi A
Molecular and cellular biochemistry 2017 Jan;424(1-2):99-110

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TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.

Huang SC, Wei PC, Hwang-Verslues WW, Kuo WH, Jeng YM, Hu CM, Shew JY, Huang CS, Chang KJ, Lee EY, Lee WH
EMBO molecular medicine 2017 Dec;9(12):1660-1680

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Expression of USP15, TβR-I and Smad7 in psoriasis.

Feng AP, He YM, Liu XX, Li JW, Tu YT, Hu F, Chen SJ
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2014 Jun;34(3):415-419

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siRNA-targeting transforming growth factor-β type I receptor reduces wound scarring and extracellular matrix deposition of scar tissue.

Wang YW, Liou NH, Cherng JH, Chang SJ, Ma KH, Fu E, Liu JC, Dai NT
The Journal of investigative dermatology 2014 Jul;134(7):2016-2025

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 μg of whole cell lysate) A: A431 B: HeLa C: HepG2 7.5% SDS PAGE GTX102784 diluted at 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

TGF beta Receptor I antibody detects TGFBR1 protein by Western blot analysis.A. 30 μg PC-12 whole cell lysate/extract7.5 % SDS-PAGETGF beta Receptor I antibody (GTX102784) dilution: 1:10000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

TGF beta Receptor I antibody detects TGF beta Receptor I protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TGF beta Receptor I antibody (GTX102784) diluted by 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed HeLa, using TGF beta Receptor I(GTX102784) antibody at 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

TGF beta Receptor I antibody detects TGF beta Receptor I protein at cytosol and membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. TGF beta Receptor I antibody (GTX102784) dilution: 1:500.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

TGF beta Receptor I antibody (GTX102784) detects TGF beta Receptor I protein by flow cytometry analysis.Sample: HepG2 cell fixed in 4% paraformaldehyde at 4°C for 15 min.Black: Unlabelled sample was used as a control.Red: TGF beta Receptor I antibody (GTX102784) dilution: 1:50.Acquisition of 20,000 events were collected using a Dylight 488-conjμgated secondary antibody for FACS analysis.