LS-C815139

antibody from LSBio

Targeting: HSPB7 cvHSP

Western blot (Enhanced validation)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C815139

Provider

LSBio

Product name

HSPB7 / CvHSP Antibody (Preservative Free) LS-C815139

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-Hspb7 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse heart tissue lysate Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa. (We are unsure as to the identity of these extra bands.)

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Hspb7 was immunoprecipitated using: Lane A: 0.5 mg NIH-3T3 Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. 4 uL anti-Hspb7 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-Hspb7 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 22 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Hspb7 was immunoprecipitated using: Lane A: 0.5 mg NIH-3T3 Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. 4 uL anti-Hspb7 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-Hspb7 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 22 kDa.