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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [5]
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- Product number
- NBP2-30673 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal BAF180/PB1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Specificity of human BAF180/PB1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-PBRM1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Lane 1: Marker [kDa] Lane 2: Human cell line RT-4
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining of human kidney cancer shows moderate to strong nuclear positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining of human endometrium shows strong nuclear positivity in glandular and stromal cells
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining in human endometrium and skeletal muscle tissues. Corresponding PBRM1 RNA-seq data are presented for the same tissues.
