Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [3]
- Immunohistochemistry [3]
- Flow cytometry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A700-019 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PBRM1 Recombinant Rabbit Monoclonal Antibody (BL-39-2C3)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Antibody clone number
- BL-39-2C3
- Concentration
- 0.25 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PBRM1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (Product # A700-019 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody (Product # A304-523A).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse PBRM1 by western blot. Samples: Whole cell lysate (50 µg) from BW5147.3, EL4, mIMCD-3, RenCa, NIH 3T3, TCMK-1, C2C12, A20, Ba/F3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (Product # A700-019 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody (Product # A304-523A).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PBRM1 by WB of immunoprecipitates from 293T lysate. Antibodies: Rabbit anti-PBRM1 recombinant monoclonal [BL-39-2C3] (A700-019). Secondary: ReliaBLOT® reagents (WB120).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PBRM1 by WB in HeLa, 293T, MCF7, HepG2, A549, SW620, SK-MEL28 (SK), Jurkat, and TCMK lysate. Antibody: Rabbit anti-PBRM1 recombinant monoclonal [BL-39-2C3] (A700-019). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PBRM1 in FFPE Jurkat cells by ICC. Antibody: Rabbit anti-PBRM1 recombinant monoclonal [BL-39-2C3] (A700-019). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PBRM1 in FFPE MCF7 cells by immunocytochemistry. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (Product # A700-019 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PBRM1 in FFPE Jurkat cells by ICC. Antibody: Rabbit anti-PBRM1 recombinant monoclonal [BL-39-2C3] (A700-019). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PBRM1 in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (Product # A700-019 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PBRM1 in FFPE breast by immunohistochemistry-IF. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (Product # A700-019 Lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PBRM1 in FFPE human breast by IHC. Antibody: Rabbit anti-PBRM1 recombinant monoclonal [BL-39-2C3] (A700-019). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PBRM1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-PBRM1 recombinant monoclonal [BL-39-2C3] (Product # A700-019; lot 4) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PBRM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (Product # A700-019 lot 4) used for IP at 5 µL/mg lysate. PBRM1 was also immunoprecipitated by a previous lot of this antibody (lot A700-019-3). For blotting immunoprecipitated PBRM1 (Product # A700-019) was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.
