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LearnPA5-53704
antibody from Invitrogen Antibodies
Targeting: CCDC186
C10orf118, CCCP-1, FLJ10188, FLJ35301, golgin104
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-53704 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C10orf118 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LKRTFKEGMD ELRTLRTKVK CLEDERLRTE DELSKYKEII NRQKAEIQNL LDKVKTADQL QEQLQRGKQE IENLKEEVES LNSLINDLQK DIEGSRKRES ELLLFTERLT SKNAQLQSES NSLQSQFDKV SC Highest antigen sequence identity to the following orthologs: Mouse - 89%, Rat - 86%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The Secreted Protein C10orf118 Is a New Regulator of Hyaluronan Synthesis Involved in Tumour-Stroma Cross-Talk.
Caon I, D'Angelo ML, Bartolini B, Caravà E, Parnigoni A, Contino F, Cancemi P, Moretto P, Karamanos NK, Passi A, Vigetti D, Karousou E, Viola M
Cancers 2021 Mar 5;13(5)
Cancers 2021 Mar 5;13(5)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C10orf118 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4. Samples were probed using a C10orf118 Polyclonal Antibody (Product # PA5-53704).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C10orf118 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a C10orf118 Polyclonal Antibody (Product # PA5-53704).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of C10orf118 in human cell line A-431 shows positivity in the Golgi apparatus. Samples were probed using a C10orf118 Polyclonal Antibody (Product # PA5-53704).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of C10orf118 in human duodenum tissue shows strong cytoplasmic positivity in glandular cells. Samples were probed using a C10orf118 Polyclonal Antibody (Product # PA5-53704).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Endogenous c10orf118 localizes in the Golgi apparatus in MCF-7 cells. ( A ) Confocal microscopy images of MCF-7 cells stained for c10orf118 (green) and Golgin-97 (red), a Golgi apparatus marker. ( B ) Confocal microscopy images of MCF-7 cells stained for c10orf118 (green) and calnexin (red), a specific endoplasmic reticulum marker. ( C ) Line-scan of the staining performed in the section marked with a green line in the merged view and relative quantification. Overlap of the two signals is apparent. ( D ) Line-scan of the staining in the section marked with a green line in the merged view and relative quantification. ( E ) Confocal microscopy images of MCF-7 cells nucleofected with 30 nmol of scrambled siRNA and siRNA against c10orf118 for 48 h and stained with antibody against c10orf118 (green) or Golgin-97 (red). White bars, 20 um.
