Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-27358 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fibrillin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa. Predicted reactivity: Mouse (92%), Rat (90%), Dog (92%), Pig (95%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Pro-Fibrotic Phenotype in a Patient with Segmental Stiff Skin Syndrome via TGF-β Signaling Overactivation.
Fusco C, Nardella G, Augello B, Boccafoschi F, Palumbo O, Fusaro L, Notarangelo A, Barbano R, Parrella P, Annicchiarico G, De Meco C, Micale L, Graziano P, Castori M
International journal of molecular sciences 2020 Jul 20;21(14)
International journal of molecular sciences 2020 Jul 20;21(14)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Fibrillin 1 Polyclonal Antibody (Product # PA5-27358). Sample (30 µg of whole cell lysate). Lane A: HeLa. 5% SDS PAGE. Fibrillin-1 antibody. Fibrillin 1 Polyclonal Antibody (Product # PA5-27358) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Immunohistochemistry and Fibrillin 1 gene ( FBN1 ) analysis. Dermal histology: ( A ) diffuse sclerosis of the dermis and subcutaneous fibroadipose tissue (hematoxylin-eosin staining, H&E X1.5); ( B ) collagen fibers deposition into subcutanous adipose tissue highlighted by trichrome staining and immunoreactivity for anti-CD34 Ab (X1.5). ( C ) Quantitative Real Time PCR (qPCR) was performed to measure FBN1 expression in patient and controls. Scale bars represent standard errors. ** p < 0.03, Student's t -test. Ctrl, control; Pt, patient. ( D ) Confocal study showed an excessive microfibrillar aggregates in patient's fibroblasts compared to controls by staining with anti-fibrillin 1 antibody (Ab). The panel reports only one representative image from one of the two control lines. Scale bar = 1 mum (white line). ( E ) Immunostaining with anti-fibrillin Ab (X1.5 and X10 objectives) displayed collagen fibers expression in patient's skin biopsy. Arrows indicate the staining with anti-fibrillin Ab.