Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-81178 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-81178, RRID:AB_11003398
- Product name
- Rabbit Polyclonal SLFN5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SLFN5 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Role of interferon {alpha} (IFN{alpha})-inducible Schlafen-5 in regulation of anchorage-independent growth and invasion of malignant melanoma cells.
Katsoulidis E, Mavrommatis E, Woodard J, Shields MA, Sassano A, Carayol N, Sawicki KT, Munshi HG, Platanias LC
The Journal of biological chemistry 2010 Dec 17;285(51):40333-41
The Journal of biological chemistry 2010 Dec 17;285(51):40333-41
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SLFN5 Antibody [NBP1-81178] - Simple Western lane view shows a specific band for SLFN5 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SLFN5 Antibody [NBP1-81178] - Electropherogram image(s) of corresponding Simple Western lane view. SLFN5 antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SLFN5 Antibody [NBP1-81178] - Analysis in human cell lines SK-MEL-30 and Caco-2. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178] - Staining of human bone marrow shows strong nuclear positivity in subsets of hematopoietic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178] - Staining of human endometrium shows strong nuclear positivity in glandular cells.A
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178] - Staining of human skeletal muscle shows weak nuclear positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.