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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- H00055611-M09 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00055611-M09, RRID:AB_1112541
- Product name
- OTUB1 monoclonal antibody (M09), clone 1C12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant OTUB1.
- Antigen sequence
MAAEEPQQQKQEPLGSDSEGVNCLAYDEANMAQQD
RIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQ
KIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALL
DDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHN
TFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYL
RLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEP
MCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTN
PHIFPEGSEPKVYLLYRPGHYDILYK- Isotype
- IgG
- Antibody clone number
- 1C12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Post-translational modification of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion.
Edelmann MJ, Kramer HB, Altun M, Kessler BM
The FEBS journal 2010 Jun;277(11):2515-30
The FEBS journal 2010 Jun;277(11):2515-30
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- OTUB1 monoclonal antibody (M09), clone 1C12. Western Blot analysis of OTUB1 expression in Hela S3 NE ( Cat # L013V3 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged OTUB1 is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
