PA5-58684

antibody from Invitrogen Antibodies

Targeting: OTUB1 FLJ20113, FLJ40710

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-58684

Provider

Invitrogen Antibodies

Product name

OTUB1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: AAEEPQQQKQ EPLGSDSEGV NCLAYDEAIM AQQDRIQQEI AVQNPLVSER LELSVLYKEY AEDDNIYQQK IKDLHKKYSY IRKTRPD Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

OTUB1 inhibits CNS autoimmunity by preventing IFN-γ-induced hyperactivation of astrocytes.

Wang X, Mulas F, Yi W, Brunn A, Nishanth G, Just S, Waisman A, Brück W, Deckert M, Schlüter D
The EMBO journal 2019 May 15;38(10)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of OTUB1 in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a OTUB1 Polyclonal Antibody (Product # PA5-58684). Remaining relative intensity is presented. Loading control: Anti-PPIB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of OTUB1 in mouse cell line NIH-3T3 and rat cell line NBT-II using a OTUB1 Polyclonal Antibody (Product # PA5-58684).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of OTUB1 in Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10, Lane 2: Mouse Cerebral Cortex tissue using a OTUB1 Polyclonal Antibody (Product # PA5-58684).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of OTUB1 in human cell line U-2 OS shows positivity in mitochondria. Samples were probed using an OTUB1 Polyclonal Antibody (Product # PA5-58684).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of OTUB1 in mouse olfactory bulb shows nuclear immunoreactivity in neurons. Samples were probed using an OTUB1 Polyclonal Antibody (Product # PA5-58684).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of OTUB1 in mouse cingulate cortex shows nuclear positivity in neurons. Samples were probed using an OTUB1 Polyclonal Antibody (Product # PA5-58684).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of OTUB1 in mouse hippocampus shows neuronal positivity. Samples were probed using an OTUB1 Polyclonal Antibody (Product # PA5-58684).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of OTUB1 in mouse dorsal raphe nucleus shows immunoreactivity in neurons. Samples were probed using an OTUB1 Polyclonal Antibody (Product # PA5-58684).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of OTUB1 in mouse medulla shows nuclear staining in the lateral paragigantocellular nucleus area. Samples were probed using an OTUB1 Polyclonal Antibody (Product # PA5-58684).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of OTUB1 in mouse hippocampus shows neuronal positivity. Samples were probed using an OTUB1 Polyclonal Antibody (Product # PA5-58684).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of OTUB1 in human cerebral cortex tissue shows moderate positivity in neuropil. Samples were probed using an OTUB1 Polyclonal Antibody (Product # PA5-58684).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of OTUB1 in human testis tissue shows strong cytoplasmic positivity in Leydig cells and subset of cells in seminiferus ducts. Samples were probed using an OTUB1 Polyclonal Antibody (Product # PA5-58684).