NBP1-49934

antibody from Novus Biologicals

Targeting: OTUB1 FLJ20113, FLJ40710

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NBP1-49934

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-49934, RRID:AB_10012124

Product name

Rabbit Polyclonal Otubain-1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13.

Mulas F, Wang X, Song S, Nishanth G, Yi W, Brunn A, Larsen PK, Isermann B, Kalinke U, Barragan A, Naumann M, Deckert M, Schlüter D
Cellular & molecular immunology 2021 Jun;18(6):1512-1527

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OTUB1 inhibits CNS autoimmunity by preventing IFN-γ-induced hyperactivation of astrocytes.

Wang X, Mulas F, Yi W, Brunn A, Nishanth G, Just S, Waisman A, Brück W, Deckert M, Schlüter D
The EMBO journal 2019 May 15;38(10)

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Otubain-1 Antibody [NBP1-49934] - Detection of Human and Mouse OTUB1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-OTUB1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. OTUB1 was also immunoprecipitated by rabbit anti-OTUB1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated OTUB1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).