702609

AVPR1A antibody from Invitrogen Antibodies

AVPR1

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Data presented on provider website

Immunohistochemistry

Data presented on provider website

Provider product page for 702609

Antibody Data

Product number

702609

Provider

Invitrogen Antibodies

Product name

Anti-AVPR1A Recombinant Rabbit Monoclonal Antibody (7H23L17)

Provider product page

Antibody type

Other

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Rabbit, Cat. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

7H23L17

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on HEK-293 cells stably transfected with human AVPR1A (Lane 2) and mock (Lane 1). Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. The blot was probed with Anti-AVPR1A Recombinant Rabbit Monoclonal Antibody (Product # 702609, 1 µg/mL, 1:500 dilution). An ~60-80 kDa band corresponding to AVPR1A was observed only in the transfected cell line.