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Learn11397-1-AP
antibody from Proteintech Group
Targeting: SEPTIN2
DIFF6, hNedd5, KIAA0158, NEDD5, Pnutl3, SEPT2
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [35]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- 11397-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11397-1-AP, RRID:AB_2239110
- Product name
- Septin 2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated Septin 2 antibody (Cat. #11397-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references CDC42-Effector Proteins Regulate Higher Order Structure of Septins Required for CNS Myelin Integrity.
Shigella flexneri evades septin-mediated cell-autonomous immunity via protein ADP-riboxanation.
Local RhoA activation induces septin recruitment.
Loss of Eml1 alters microtubule-associated protein networks in mouse brain heterotopia.
Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome.
Maternal PRDM10 activates essential genes for oocyte-to-embryo transition.
The Actin Motor Protein Myosin 6 Contributes to Cell Migration and Expression of GIPC1 and Septins in Breast Cancer Cells.
BET degrader exhibits lower antiproliferative activity than its inhibitor via EGR1 recruiting septins to promote E2F1-3 transcription in triple-negative breast cancer.
TMEM232 is required for the formation of sperm flagellum and male fertility in mice.
The septin modifier, forchlorfenuron, activates NLRP3 via a potassium-independent mitochondrial axis.
Borg5 restricts contractility and motility in epithelial MDCK cells.
CFTR and PC2, partners in the primary cilia in autosomal dominant polycystic kidney disease.
Targeted inactivation of the Septin2 and Septin9 genes in myelinating Schwann cells of mice.
SARS-CoV-2 ORF10 impairs cilia by enhancing CUL2ZYG11B activity.
Pinch2 regulates myelination in the mouse central nervous system.
JMJD2C-mediated long non-coding RNA MALAT1/microRNA-503-5p/SEPT2 axis worsens non-small cell lung cancer.
An anillin-CIN85-SEPT9 complex promotes intercellular bridge maturation required for successful cytokinesis.
An endometrial organoid model of interactions between Chlamydia and epithelial and immune cells.
MicroRNA‑744‑5p is downregulated in colorectal cancer and targets SEPT2 to suppress the malignant phenotype.
Revised subunit order of mammalian septin complexes explains their in vitro polymerization properties.
Rpgrip1l controls ciliary gating by ensuring the proper amount of Cep290 at the vertebrate transition zone.
The septin cytoskeleton regulates natural killer cell lytic granule release.
The SEPT12 complex is required for the establishment of a functional sperm head-tail junction.
Anillin facilitates septin assembly to prevent pathological outfoldings of central nervous system myelin.
A Septin Cytoskeleton-Targeting Small Molecule, Forchlorfenuron, Inhibits Epithelial Migration via Septin-Independent Perturbation of Cellular Signaling.
Septin-regulated actin dynamics promote Salmonella invasion of host cells.
Wiskott-Aldrich syndrome protein regulates autophagy and inflammasome activity in innate immune cells.
SEPT12 phosphorylation results in loss of the septin ring/sperm annulus, defective sperm motility and poor male fertility.
The requirement of SEPT2 and SEPT7 for migration and invasion in human breast cancer via MEK/ERK activation.
Septin/anillin filaments scaffold central nervous system myelin to accelerate nerve conduction.
SEPT12 orchestrates the formation of mammalian sperm annulus by organizing core octameric complexes with other SEPT proteins.
Septins arrange F-actin-containing fibers on the Chlamydia trachomatis inclusion and are required for normal release of the inclusion by extrusion.
FHL2-driven molecular network mediated Septin2 knockdown inducing apoptosis in mesangial cell.
Genetic deletion of SEPT7 reveals a cell type-specific role of septins in microtubule destabilization for the completion of cytokinesis.
Deciphering the rules governing assembly order of mammalian septin complexes.
Hümmert S, de Faria JP, Jahn O, Bilgin E, Łukasik N, Rao C, Mitkovski M, Benseler F, Brose N, Siems SB, Goebbels S, Möbius W, Ewers H, Relvas JB, Werner HB
Glia 2026 Mar;74(3):e70134
Glia 2026 Mar;74(3):e70134
Shigella flexneri evades septin-mediated cell-autonomous immunity via protein ADP-riboxanation.
Tang Z, Xian W, Özbaykal Güler G, Zhang H, Wang Y, Zhan N, Liu H, You F, Zou Q, Mostowy S, Liu X
Nature communications 2026 Jan 27;17(1):1727
Nature communications 2026 Jan 27;17(1):1727
Local RhoA activation induces septin recruitment.
Chandrasekar S, Utgaard ME, Somerfield B, Wu H, Beach JR, Oakes PW
bioRxiv : the preprint server for biology 2025 Sep 30;
bioRxiv : the preprint server for biology 2025 Sep 30;
Loss of Eml1 alters microtubule-associated protein networks in mouse brain heterotopia.
Yigit BN, Tufekci D, Viola V, Kirim BA, Zaidi D, Francis F, Ozlu N
Communications biology 2025 Jul 3;8(1):989
Communications biology 2025 Jul 3;8(1):989
Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome.
Siems SB, Gargareta VI, Schadt LC, Daguano Gastaldi V, Jung RB, Piepkorn L, Casaccia P, Sun T, Jahn O, Werner HB
Glia 2025 Jan;73(1):38-56
Glia 2025 Jan;73(1):38-56
Maternal PRDM10 activates essential genes for oocyte-to-embryo transition.
Seah MKY, Han BY, Huang Y, Rasmussen LJH, Stäubli AJ, Bello-Rodríguez J, Chan AC, Gasnier M, Wollmann H, Guccione E, Messerschmidt DM
Nature communications 2025 Feb 24;16(1):1939
Nature communications 2025 Feb 24;16(1):1939
The Actin Motor Protein Myosin 6 Contributes to Cell Migration and Expression of GIPC1 and Septins in Breast Cancer Cells.
Izdebska M, Arendt W, Hałas-Wiśniewska M, Zakrzewski P, Lenartowski R, Lenartowska M
Cancer management and research 2024;16:1445-1462
Cancer management and research 2024;16:1445-1462
BET degrader exhibits lower antiproliferative activity than its inhibitor via EGR1 recruiting septins to promote E2F1-3 transcription in triple-negative breast cancer.
Liu N, Wang S, Li M, Zhao N, Wang D, Zhang R, Yu M, Zhao L, Zhang S, Han F, Zhao Y, Liu Q
Pharmacological research 2024 Oct;208:107377
Pharmacological research 2024 Oct;208:107377
TMEM232 is required for the formation of sperm flagellum and male fertility in mice.
Cai X, Zhang H, Kong S, Xu W, Zheng J, Wang N, He S, Li S, Shen Y, Wang K, Zhang Z, Cai H, Ma F, Bai S, Zhu F, Xiao F, Wang F
Cell death & disease 2024 Nov 8;15(11):806
Cell death & disease 2024 Nov 8;15(11):806
The septin modifier, forchlorfenuron, activates NLRP3 via a potassium-independent mitochondrial axis.
Holley CL, Emming S, Monteleone MM, Mellacheruvu M, Kenney KM, Lawrence GMEP, Coombs JR, Burgener SS, Schroder K
Cell chemical biology 2024 May 16;31(5):962-972.e4
Cell chemical biology 2024 May 16;31(5):962-972.e4
Borg5 restricts contractility and motility in epithelial MDCK cells.
Cohen D, Fernandez D, Lázaro-Diéguez F, Überheide B, Müsch A
Journal of cell science 2024 Dec 1;137(23)
Journal of cell science 2024 Dec 1;137(23)
CFTR and PC2, partners in the primary cilia in autosomal dominant polycystic kidney disease.
Yanda MK, Ciobanu C, Guggino WB, Cebotaru L
American journal of physiology. Cell physiology 2023 Sep 1;325(3):C682-C693
American journal of physiology. Cell physiology 2023 Sep 1;325(3):C682-C693
Targeted inactivation of the Septin2 and Septin9 genes in myelinating Schwann cells of mice.
Martens AK, Erwig M, Patzig J, Fledrich R, Füchtbauer EM, Werner HB
Cytoskeleton (Hoboken, N.J.) 2023 Jul-Aug;80(7-8):290-302
Cytoskeleton (Hoboken, N.J.) 2023 Jul-Aug;80(7-8):290-302
SARS-CoV-2 ORF10 impairs cilia by enhancing CUL2ZYG11B activity.
Wang L, Liu C, Yang B, Zhang H, Jiao J, Zhang R, Liu S, Xiao S, Chen Y, Liu B, Ma Y, Duan X, Guo Y, Guo M, Wu B, Wang X, Huang X, Yang H, Gui Y, Fang M, Zhang L, Duo S, Guo X, Li W
The Journal of cell biology 2022 Jul 4;221(7)
The Journal of cell biology 2022 Jul 4;221(7)
Pinch2 regulates myelination in the mouse central nervous system.
Paes de Faria J, Vale-Silva RS, Fässler R, Werner HB, Relvas JB
Development (Cambridge, England) 2022 Jul 1;149(13)
Development (Cambridge, England) 2022 Jul 1;149(13)
JMJD2C-mediated long non-coding RNA MALAT1/microRNA-503-5p/SEPT2 axis worsens non-small cell lung cancer.
Zhang J, Wang M, Wang J, Wang W
Cell death & disease 2022 Jan 19;13(1):65
Cell death & disease 2022 Jan 19;13(1):65
An anillin-CIN85-SEPT9 complex promotes intercellular bridge maturation required for successful cytokinesis.
Panagiotou TC, Chen A, Wilde A
Cell reports 2022 Aug 30;40(9):111274
Cell reports 2022 Aug 30;40(9):111274
An endometrial organoid model of interactions between Chlamydia and epithelial and immune cells.
Dolat L, Valdivia RH
Journal of cell science 2021 Mar 8;134(5)
Journal of cell science 2021 Mar 8;134(5)
MicroRNA‑744‑5p is downregulated in colorectal cancer and targets SEPT2 to suppress the malignant phenotype.
Zhang W, Liao K, Liu D
Molecular medicine reports 2021 Jan;23(1)
Molecular medicine reports 2021 Jan;23(1)
Revised subunit order of mammalian septin complexes explains their in vitro polymerization properties.
Soroor F, Kim MS, Palander O, Balachandran Y, Collins RF, Benlekbir S, Rubinstein JL, Trimble WS
Molecular biology of the cell 2021 Feb 1;32(3):289-300
Molecular biology of the cell 2021 Feb 1;32(3):289-300
Rpgrip1l controls ciliary gating by ensuring the proper amount of Cep290 at the vertebrate transition zone.
Wiegering A, Dildrop R, Vesque C, Khanna H, Schneider-Maunoury S, Gerhardt C
Molecular biology of the cell 2021 Apr 15;32(8):675-689
Molecular biology of the cell 2021 Apr 15;32(8):675-689
The septin cytoskeleton regulates natural killer cell lytic granule release.
Phatarpekar PV, Overlee BL, Leehan A, Wilton KM, Ham H, Billadeau DD
The Journal of cell biology 2020 Nov 2;219(11)
The Journal of cell biology 2020 Nov 2;219(11)
The SEPT12 complex is required for the establishment of a functional sperm head-tail junction.
Shen YR, Wang HY, Tsai YC, Kuo YC, Wu SR, Wang CY, Kuo PL
Molecular human reproduction 2020 Jun 1;26(6):402-412
Molecular human reproduction 2020 Jun 1;26(6):402-412
Anillin facilitates septin assembly to prevent pathological outfoldings of central nervous system myelin.
Erwig MS, Patzig J, Steyer AM, Dibaj P, Heilmann M, Heilmann I, Jung RB, Kusch K, Möbius W, Jahn O, Nave KA, Werner HB
eLife 2019 Jan 23;8
eLife 2019 Jan 23;8
A Septin Cytoskeleton-Targeting Small Molecule, Forchlorfenuron, Inhibits Epithelial Migration via Septin-Independent Perturbation of Cellular Signaling.
Sun L, Cao X, Lechuga S, Feygin A, Naydenov NG, Ivanov AI
Cells 2019 Dec 29;9(1)
Cells 2019 Dec 29;9(1)
Septin-regulated actin dynamics promote Salmonella invasion of host cells.
Boddy KC, Gao AD, Truong D, Kim MS, Froese CD, Trimble WS, Brumell JH
Cellular microbiology 2018 Oct;20(10):e12866
Cellular microbiology 2018 Oct;20(10):e12866
Wiskott-Aldrich syndrome protein regulates autophagy and inflammasome activity in innate immune cells.
Lee PP, Lobato-Márquez D, Pramanik N, Sirianni A, Daza-Cajigal V, Rivers E, Cavazza A, Bouma G, Moulding D, Hultenby K, Westerberg LS, Hollinshead M, Lau YL, Burns SO, Mostowy S, Bajaj-Elliott M, Thrasher AJ
Nature communications 2017 Nov 17;8(1):1576
Nature communications 2017 Nov 17;8(1):1576
SEPT12 phosphorylation results in loss of the septin ring/sperm annulus, defective sperm motility and poor male fertility.
Shen YR, Wang HY, Kuo YC, Shih SC, Hsu CH, Chen YR, Wu SR, Wang CY, Kuo PL
PLoS genetics 2017 Mar;13(3):e1006631
PLoS genetics 2017 Mar;13(3):e1006631
The requirement of SEPT2 and SEPT7 for migration and invasion in human breast cancer via MEK/ERK activation.
Zhang N, Liu L, Fan N, Zhang Q, Wang W, Zheng M, Ma L, Li Y, Shi L
Oncotarget 2016 Sep 20;7(38):61587-61600
Oncotarget 2016 Sep 20;7(38):61587-61600
Septin/anillin filaments scaffold central nervous system myelin to accelerate nerve conduction.
Patzig J, Erwig MS, Tenzer S, Kusch K, Dibaj P, Möbius W, Goebbels S, Schaeren-Wiemers N, Nave KA, Werner HB
eLife 2016 Aug 9;5
eLife 2016 Aug 9;5
SEPT12 orchestrates the formation of mammalian sperm annulus by organizing core octameric complexes with other SEPT proteins.
Kuo YC, Shen YR, Chen HI, Lin YH, Wang YY, Chen YR, Wang CY, Kuo PL
Journal of cell science 2015 Mar 1;128(5):923-34
Journal of cell science 2015 Mar 1;128(5):923-34
Septins arrange F-actin-containing fibers on the Chlamydia trachomatis inclusion and are required for normal release of the inclusion by extrusion.
Volceanov L, Herbst K, Biniossek M, Schilling O, Haller D, Nölke T, Subbarayal P, Rudel T, Zieger B, Häcker G
mBio 2014 Oct 7;5(5):e01802-14
mBio 2014 Oct 7;5(5):e01802-14
FHL2-driven molecular network mediated Septin2 knockdown inducing apoptosis in mesangial cell.
Lu Y, Cai G, Cui S, Geng W, Chen D, Wen J, Zhang Y, Zhang F, Xie Y, Fu B, Chen X
Proteomics 2014 Nov;14(21-22):2485-97
Proteomics 2014 Nov;14(21-22):2485-97
Genetic deletion of SEPT7 reveals a cell type-specific role of septins in microtubule destabilization for the completion of cytokinesis.
Menon MB, Sawada A, Chaturvedi A, Mishra P, Schuster-Gossler K, Galla M, Schambach A, Gossler A, Förster R, Heuser M, Kotlyarov A, Kinoshita M, Gaestel M
PLoS genetics 2014 Aug;10(8):e1004558
PLoS genetics 2014 Aug;10(8):e1004558
Deciphering the rules governing assembly order of mammalian septin complexes.
Sellin ME, Sandblad L, Stenmark S, Gullberg M
Molecular biology of the cell 2011 Sep;22(17):3152-64
Molecular biology of the cell 2011 Sep;22(17):3152-64
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 11397-1-AP(SEPT2 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using SEPT2 antibody 113971-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-SEPT2 (IP:11397-1-AP, 3ug; Detection:11397-1-AP 1:1000) with mouse brain tissue lysate 5000ug.
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human pancreas cancer using 11397-1-AP(SEPT2 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The SEPT2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SEPT2. This antibody recognizes human,mouse,rat antigen. The SEPT2 antibody has been validated for the following applications: ELISA, IHC, IP, IF, WB, FC analysis.
