MA5-14884

antibody from Invitrogen Antibodies

Targeting: KAT2A GCN5, GCN5L2, PCAF-b

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA5-14884

Provider

Invitrogen Antibodies

Product name

GCN5 Monoclonal Antibody (S.775.4)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with the related PCAF protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

S.775.4

Vial size

100 µL

Concentration

134 µg/mL

Storage

-20°C

References

Submitted references

LINC00842 inactivates transcription co-regulator PGC-1α to promote pancreatic cancer malignancy through metabolic remodelling.

Huang X, Pan L, Zuo Z, Li M, Zeng L, Li R, Ye Y, Zhang J, Wu G, Bai R, Zhuang L, Wei L, Zheng Y, Su J, Deng J, Deng S, Zhang S, Zhu S, Che X, Wang C, Wu C, Chen R, Lin D, Zheng J
Nature communications 2021 Jun 22;12(1):3830

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of GCN5 was achieved by transfecting HeLa cells with GCN5 specific siRNAs (Silencer® select Product # s5657). Western blot analysis (Fig. a) was performed using whole cell extracts from the GCN5 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with GCN5 Monoclonal Antibody (Product # MA5-14884, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to GCN5.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts of Hep G2 (Lane 1), Raji (Lane 2), Ramos (Lane 3), U-2OS (Lane 4), A549 (Lane 5), SH-SY5Y (Lane 6), HeLa (Lane 7) and HCT 116 (Lane 8). The blot was probed with Anti-GCN5 Monoclonal Antibody (S.775.4) (Product # MA5-14884, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 94 kDa band corresponding to GCN5 was observed across the cell lines tested.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GCN5L2 in HeLa cells using a GCN5L2 monoclonal antibody (Product # MA5-14884) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.