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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-47222 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BMP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TLL-2 is observed, and less than 1% cross-reactivity with rhBMP-2, -3, -3b, -4, -5, -6, -7, -8, -9, -10, and -15 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BMP1 in 25 ng recombinant Human BMP‚1/ PCP, recombinant Human TLL-2, recombinant Human/Mouse/Rat BMP‚2, recombinant Human BMP‚3, recombinant Human BMP‚3b/GDF‚10, recombinant Human BMP‚4, recombinant Human BMP‚5, recombinant Human BMP‚6, recombinant Human BMP‚7, recombinant Human BMP‚8a, recombinant Human BMP‚8b, recombinant Human BMP‚9, recombinant Human BMP‚10 and recombinant Human BMP‚15/GDF‚9B. Samples were incubated in BMP1 polyclonal antibody (Product # PA5-47222) using a dilution of 0.1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for BMP‚1/PCP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BMP1 in 25 ng recombinant Human BMP‚1/ PCP, recombinant Human TLL-2, recombinant Human Meprin α Subunit/MEP1A, recombinant Human Meprin β Subunit/MEP1B, recombinant Human Ovastacin, recombinant Human ADAM10, recombinant Human ADAMTS4, and recombinant Human MMP‚1. Samples were incubated in BMP1 polyclonal antibody (Product # PA5-47222) using a dilution of 0.1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for BMP‚1/PCP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions.
