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LearnMAB6531-100
antibody from R&D Systems
Targeting: CCL26
eotaxin-3, IMAC, MIP-4a, MIP-4alpha, SCYA26, TSC-1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- MAB6531-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL26/Eotaxin-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human CCL26/Eotaxin-3 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y258- Isotype
- IgG
- Antibody clone number
- 2152H
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CCL26/Eotaxin-3 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 U/mL Recombinant Human IL-4 (Catalog # 204-IL) for 24 hours. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human CCL26/Eotaxin-3 Monoclonal Antibody (Catalog # MAB6531) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CCL26/Eotaxin-3 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
