Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX111409 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111409, RRID:AB_10722406
- Product name
- Hexokinase III antibody [C1C3-2]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE GTX111409 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase III antibody [C1C3-2] (GTX111409) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using Hexokinase III(GTX111409) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Hexokinase III antibody [C1C3-2] detects Hexokinase III protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Hexokinase III protein stained by Hexokinase III antibody [C1C3-2] (GTX111409) diluted at 1:500.Blue: Hoechst 33342 staining.
