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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BS1485 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS1485, RRID:AB_1663923
- Product name
- SAE2 (S621) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 598-640 of Human SAE2.
- Description
- The human UBiquitin Activating domain 2 (UBA2) gene is located on chromosome 19q12. Human UBA2 could form a beta mercaptoethanol-sensitive conjugate with members of the sentrin family, but not with ubiquitin of NEDD8 in the presence of AOS1. The recombinant proteins, Sua1p and hUba2, form a complex. In this complex, hUba2 binds SUMO-1, which gives the complex the activity of the SUMO-1 activating enzyme. Therefore, UBA2 has been found to be a component of the sentrin-activating enzyme complex.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of SAE2 (S621) pAb at 1:500 dilutionLane1:A549 whole cell lysateLane2:Raw264.7 whole cell lysateLane3:Rat heart tissue lysate
