Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB2233 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2233, RRID:AB_1580342
- Product name
- UBA2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of UBA2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2.
Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.
Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex.
Okuma T, Honda R, Ichikawa G, Tsumagari N, Yasuda H
Biochemical and biophysical research communications 1999 Jan 27;254(3):693-8
Biochemical and biophysical research communications 1999 Jan 27;254(3):693-8
Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.
Desterro JM, Rodriguez MS, Kemp GD, Hay RT
The Journal of biological chemistry 1999 Apr 9;274(15):10618-24
The Journal of biological chemistry 1999 Apr 9;274(15):10618-24
Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex.
Gong L, Li B, Millas S, Yeh ET
FEBS letters 1999 Apr 1;448(1):185-9
FEBS letters 1999 Apr 1;448(1):185-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The UBA2 polyclonal antibody (Cat # PAB2233) is used in Western blot to detect UBA2 in Jurkat lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the UBA2 polyclonal antibody (Cat # PAB2233) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)