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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 15347-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: TNASDQQNE PQLGLKDQQV LDVKSYARLF SKSIETLRVH LAEKGDGAEL IWDKDDPSAM DFVTSAANLR MHIFSMNMKS RFDIKSMAGN IIPAIATTNA VIAGLIVLEG LKILSGKIDQ CRTIFLNKQP NPRKKLLVPC ALDPPNPNCY VCASKPEVTV RLNVHKVTVL TLQDKIVKEK FAMVAPDVQI EDGKGTILIS SEEGETEANN HKKLSEFGIR NGSRLQADDF LQDYTLLINI LHSEDLGKDV EFEVVGDAPE KVGPKQAEDA AKSITNGSDD GAQPSTSTAQ EQDDVLIVDS DEEDSSNNAD VSEEERSRKR KLDEKENLSA KRSRIEQKEE LDDVIALD (294-640 aa encoded by BC003153)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 15347-1-AP (UBA2 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 15347-1-AP (UBA2 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 15347-1-AP (UBA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 15347-1-AP (UBA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 15347-1-AP (UBA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-UBA2 (IP:15347-1-AP, 4ug; Detection:15347-1-AP 1:500) with Jurkat cells lysate 3200ug.
