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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
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- Product number
- PA5-57978 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAP80 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PVFENVNVKS FDRCTGHSAE HTQCGKPQES TGRGSAFLKA VQGSGDTSRH CLPTLADAKG LQDTGGTVNY FWGIPFCPDG VDPNQYTKVI LCQLE Highest antigen sequence identity to the following orthologs: Mouse - 84%, Rat - 81%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAP80 in control (vector only transfected HEK293T lysate) and UIMC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a RAP80 Polyclonal Antibody (Product # PA5-57978).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RAP80 in human cell line U-251 MG shows positivity in nucleus. Samples were probed using a RAP80 Polyclonal Antibody (Product # PA5-57978).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RAP80 in human cell line U-2 OS using a RAP80 Polyclonal Antibody (Product # PA5-57978) shows localization to nuclear bodies.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RAP80 in human lymph node using RAP80 Polyclonal Antibody (Product # PA5-57978).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RAP80 in human colon using RAP80 Polyclonal Antibody (Product # PA5-57978).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RAP80 in human testis using RAP80 Polyclonal Antibody (Product # PA5-57978).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RAP80 in human cerebral cortex using RAP80 Polyclonal Antibody (Product # PA5-57978).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RAP80 in human cerebral cortex, colon, lymph node and testis using RAP80 Polyclonal Antibody (Product # PA5-57978) (A) shows similar protein distribution across tissues to an independent RAP80 Polyclonal Antibody (B).
