Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-87156 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87156, RRID:AB_10999813
- Product name
- Rabbit Polyclonal RAP80 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human RAP80 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Single-dose radiotherapy disables tumor cell homologous recombination via ischemia/reperfusion injury.
Human Rad52 Promotes XPG-Mediated R-loop Processing to Initiate Transcription-Associated Homologous Recombination Repair.
The Ubiquitin E3/E4 Ligase UBE4A Adjusts Protein Ubiquitylation and Accumulation at Sites of DNA Damage, Facilitating Double-Strand Break Repair.
MDC1 methylation mediated by lysine methyltransferases EHMT1 and EHMT2 regulates active ATM accumulation flanking DNA damage sites.
The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites.
Impaired TIP60-mediated H4K16 acetylation accounts for the aberrant chromatin accumulation of 53BP1 and RAP80 in Fanconi anemia pathway-deficient cells.
Triapine disrupts CtIP-mediated homologous recombination repair and sensitizes ovarian cancer cells to PARP and topoisomerase inhibitors.
Fine-tuning of DNA damage-dependent ubiquitination by OTUB2 supports the DNA repair pathway choice.
Bodo S, Campagne C, Thin TH, Higginson DS, Vargas HA, Hua G, Fuller JD, Ackerstaff E, Russell J, Zhang Z, Klingler S, Cho H, Kaag MG, Mazaheri Y, Rimner A, Manova-Todorova K, Epel B, Zatcky J, Cleary CR, Rao SS, Yamada Y, Zelefsky MJ, Halpern HJ, Koutcher JA, Cordon-Cardo C, Greco C, Haimovitz-Friedman A, Sala E, Powell SN, Kolesnick R, Fuks Z
The Journal of clinical investigation 2019 Feb 1;129(2):786-801
The Journal of clinical investigation 2019 Feb 1;129(2):786-801
Human Rad52 Promotes XPG-Mediated R-loop Processing to Initiate Transcription-Associated Homologous Recombination Repair.
Yasuhara T, Kato R, Hagiwara Y, Shiotani B, Yamauchi M, Nakada S, Shibata A, Miyagawa K
Cell 2018 Oct 4;175(2):558-570.e11
Cell 2018 Oct 4;175(2):558-570.e11
The Ubiquitin E3/E4 Ligase UBE4A Adjusts Protein Ubiquitylation and Accumulation at Sites of DNA Damage, Facilitating Double-Strand Break Repair.
Baranes-Bachar K, Levy-Barda A, Oehler J, Reid DA, Soria-Bretones I, Voss TC, Chung D, Park Y, Liu C, Yoon JB, Li W, Dellaire G, Misteli T, Huertas P, Rothenberg E, Ramadan K, Ziv Y, Shiloh Y
Molecular cell 2018 Mar 1;69(5):866-878.e7
Molecular cell 2018 Mar 1;69(5):866-878.e7
MDC1 methylation mediated by lysine methyltransferases EHMT1 and EHMT2 regulates active ATM accumulation flanking DNA damage sites.
Watanabe S, Iimori M, Chan DV, Hara E, Kitao H, Maehara Y
Scientific reports 2018 Jul 18;8(1):10888
Scientific reports 2018 Jul 18;8(1):10888
The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites.
Ha K, Ma C, Lin H, Tang L, Lian Z, Zhao F, Li JM, Zhen B, Pei H, Han S, Malumbres M, Jin J, Chen H, Zhao Y, Zhu Q, Zhang P
Nature communications 2017 Jun 12;8:15751
Nature communications 2017 Jun 12;8:15751
Impaired TIP60-mediated H4K16 acetylation accounts for the aberrant chromatin accumulation of 53BP1 and RAP80 in Fanconi anemia pathway-deficient cells.
Renaud E, Barascu A, Rosselli F
Nucleic acids research 2016 Jan 29;44(2):648-56
Nucleic acids research 2016 Jan 29;44(2):648-56
Triapine disrupts CtIP-mediated homologous recombination repair and sensitizes ovarian cancer cells to PARP and topoisomerase inhibitors.
Lin ZP, Ratner ES, Whicker ME, Lee Y, Sartorelli AC
Molecular cancer research : MCR 2014 Mar;12(3):381-393
Molecular cancer research : MCR 2014 Mar;12(3):381-393
Fine-tuning of DNA damage-dependent ubiquitination by OTUB2 supports the DNA repair pathway choice.
Kato K, Nakajima K, Ui A, Muto-Terao Y, Ogiwara H, Nakada S
Molecular cell 2014 Feb 20;53(4):617-30
Molecular cell 2014 Feb 20;53(4):617-30
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAP80 Antibody [NBP1-87156] - Analysis in human cell line HDLM-2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAP80 Antibody [NBP1-87156] - Analysis in control (vector only transfected HEK293T lysate) and UIMC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-87156] - Staining of human cerebral cortex using Anti-UIMC1 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-87156] - Staining of human colon using Anti-UIMC1 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-87156] - Staining of human lymph node using Anti-UIMC1 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-87156] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-UIMC1 antibody NBP1-87156 (A) shows similar protein distribution across tissues to independent antibody NBP1-87157 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-87156] - Staining of human testis.