21625-1-AP

antibody from Proteintech Group

Targeting: RNF20 BRE1, BRE1A, FLJ11189, FLJ20382, hBRE1, KAIA2779

Provider product page for 21625-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [14]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 21625-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#21625-1-AP, RRID:AB_10734436
Product name: RNF20 antibody
Antibody type: Polyclonal
Description: KD/KO validated RNF20 antibody (Cat. #21625-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

RNF20 protein structure - 21625-1-AP shown in red.

Submitted references Albendazole inhibits colon cancer progression and therapy resistance by targeting ubiquitin ligase RNF20.

Fatima I, Ahmad R, Barman S, Gowrikumar S, Pravoverov K, Primeaux M, Fisher KW, Singh AB, Dhawan P
British journal of cancer 2024 Apr;130(6):1046-1058

RNF20 Regulates Oocyte Meiotic Spindle Assembly by Recruiting TPM3 to Centromeres and Spindle Poles.

Wang L, Liu C, Li L, Wei H, Wei W, Zhou Q, Chen Y, Meng TG, Jiao R, Wang ZB, Sun QY, Li W
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Apr;11(13):e2306986

Epigenetic regulator RNF20 underlies temporal hierarchy of gene expression to regulate postnatal cardiomyocyte polarization.

Lin CY, Chang YM, Tseng HY, Shih YL, Yeh HH, Liao YR, Tang HH, Hsu CL, Chen CC, Yan YT, Kao CF
Cell reports 2023 Nov 28;42(11):113416

Adipocyte Rnf20 ablation increases the fast-twitch fibers of skeletal muscle via lysophosphatidylcholine 16:0.

Zhao Y, Chen C, Pan J, Lam SM, Shui G, Yang S, Wu T, Yang N, Tao C, Zhao J, Wang Y
Cellular and molecular life sciences : CMLS 2023 Aug 9;80(9):243

Antinuclear antibodies in individuals with COVID-19 reflect underlying disease: Identification of new autoantibodies in systemic sclerosis (CDK9) and malignancy (RNF20, RCC1, TRIP13).

Bossuyt X, Vulsteke JB, Van Elslande J, Boon L, Wuyts G, Willebrords S, Frans G, Geukens N, Carpentier S, Tejpar S, Wildiers H, Blockmans D, De Langhe E, Vermeersch P, Derua R
Autoimmunity reviews 2023 Apr;22(4):103288

RNF20 is required for male fertility through regulation of H2B ubiquitination in the Sertoli cells.

Lai F, Wang H, Zhao X, Yang K, Cai L, Hu M, Lin L, Xia X, Li W, Cheng H, Zhou R
Cell & bioscience 2023 Apr 6;13(1):71

Endothelial cells regulated by RNF20 orchestrate the proliferation and differentiation of neural precursor cells during embryonic development.

Zhang M, Su L, Wang W, Li C, Liang Q, Ji F, Jiao J
Cell reports 2022 Sep 13;40(11):111350

A siRNA screening of UBE2 family demonstrated that UBE2R1 had a high repressive effect on HIV Tat protein.

Xu M, Zhang J
Biochemistry and biophysics reports 2022 Dec;32:101366

Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis.

Liang X, Tao C, Pan J, Zhang L, Liu L, Zhao Y, Fan Y, Cao C, Liu J, Zhang J, Lam SM, Shui G, Jin W, Li W, Zhao J, Li K, Wang Y
Protein & cell 2021 Jun;12(6):475-492

RNF20 affects porcine adipocyte differentiation via regulation of mitotic clonal expansion.

Zhao Y, Pan J, Cao C, Liang X, Yang S, Liu L, Tao C, Zhao J, Wang Y
Cell proliferation 2021 Dec;54(12):e13131

Histone H2B ubiquitination mediated chromatin relaxation is essential for the induction of somatic cell reprogramming.

Wang L, Xu Z, Wang L, Liu C, Wei H, Zhang R, Chen Y, Wang L, Liu W, Xiao S, Li W, Li W
Cell proliferation 2021 Aug;54(8):e13080

Molecular Characterization, Expression Profiling, and SNP Analysis of the Porcine RNF20 Gene.

Zhao Y, Yang S, Wang Y, Tao C
Animals : an open access journal from MDPI 2020 May 20;10(5)

SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.

Xu M, Moresco JJ, Chang M, Mukim A, Smith D, Diedrich JK, Yates JR 3rd, Jones KA
PLoS pathogens 2018 May;14(5):e1007071

H2B ubiquitination regulates meiotic recombination by promoting chromatin relaxation.

Xu Z, Song Z, Li G, Tu H, Liu W, Liu Y, Wang P, Wang Y, Cui X, Liu C, Shang Y, de Rooij DG, Gao F, Li W
Nucleic acids research 2016 Nov 16;44(20):9681-9697

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 21625-1-AP(RNF20 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using RNF20 antibody 21625-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line