PA5-53108

antibody from Invitrogen Antibodies

Targeting: NECAB2 EFCBP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-53108

Provider

Invitrogen Antibodies

Product name

NECAB2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: VILDIFRRAD KNDDGKLSLE EFQLFFADGV LNEKELEDLF HTIDSDNTNH VDTKELCDYF VDHMGDYEDV LASLETLNHS VLKAMGYTKK VYEGGSNVDQ FVTRFLLKET ANQIQSLLSS VESAVEAIEE QTSQL Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 97%.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.3 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Transcriptome Profiling of the Dorsomedial Prefrontal Cortex in Suicide Victims.

Dóra F, Renner É, Keller D, Palkovits M, Dobolyi Á
International journal of molecular sciences 2022 Jun 25;23(13)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NECAB2 in human cerebral cortex tissue using a NECAB2 Polyclonal Antibody (Product # PA5-53108).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NECAB2 in mouse cerebral cortex tissue using a NECAB2 Polyclonal Antibody (Product # PA5-53108).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NECAB2 in human cell line U-2 OS shows positivity in vesicles. Samples were probed using a NECAB2 Polyclonal Antibody (Product # PA5-53108).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NECAB2 in mouse caudate putamen shows immunoreactivity in medium spiny neurons and synapses. Samples were probed using a NECAB2 Polyclonal Antibody (Product # PA5-53108).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NECAB2 in human colon using NECAB2 Polyclonal Antibody (Product # PA5-53108).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NECAB2 in human kidney using NECAB2 Polyclonal Antibody (Product # PA5-53108).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NECAB2 in human pancreas using NECAB2 Polyclonal Antibody (Product # PA5-53108).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NECAB2 in mouse hippocampus shows immunoreactivity in a subset of neurons. Samples were probed using a NECAB2 Polyclonal Antibody (Product # PA5-53108).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NECAB2 in mouse midbrain shows neuronal positivity in dorsal raphe. Samples were probed using a NECAB2 Polyclonal Antibody (Product # PA5-53108).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NECAB2 in human cerebral cortex using NECAB2 Polyclonal Antibody (Product # PA5-53108).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NECAB2 in mouse facial nucleus shows distinct positivity in neuronal cell bodies and processes. Samples were probed using a NECAB2 Polyclonal Antibody (Product # PA5-53108).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NECAB2 in human cerebral cortex, colon, kidney and pancreas using NECAB2 Polyclonal Antibody (Product # PA5-53108) (A) shows similar protein distribution across tissues to an independent NECAB2 Polyclonal Antibody (B).