HPA014144

antibody from Atlas Antibodies

Targeting: NECAB2 EFCBP2

Antibody data

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Validation data

Product number: HPA014144 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA014144, RRID:AB_1848017
Product name: Anti-NECAB2
Antibody type: Polyclonal
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: ATEDAKEEGLEAQISRLAELIGRLESKALWFDLQQ RLSDEDGTNMHLQLVRQEMAVCPEQLSEFLDSLRQ YLRGTTGVRNCFHITAVRLSDGFTFVIYEFWETEE AWKRHLQSPLCKAFRHVKVDTLSQPEALSRILVPA
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

NECAB2 protein structure - HPA014144 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-NECAB2 antibody. Corresponding NECAB2 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex, colon, kidney and pancreas using Anti-NECAB2 antibody HPA014144 (A) shows similar protein distribution across tissues to independent antibody HPA013998 (B).
Antibody #2 product nr: HPA013998
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex shows cytoplasmic positivity in neurons.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human pancreas shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human colon using Anti-NECAB2 antibody HPA014144.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney using Anti-NECAB2 antibody HPA014144.
Sample type: HUMAN