Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- HPA038469 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA038469, RRID:AB_10671743
- Product name
- Anti-RASSF3
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human RASSF3, Gene description: Ras association (RalGDS/AF-6) domain family member 3, Validated applications: ICC, IHC, WB, Uniprot ID: Q86WH2, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Complex interplay between RAS GTPases and RASSF effectors regulates subcellular localization of YAP
Singh S, Bernal Astrain G, Hincapie A, Goudreault M, Smith M
EMBO Reports 2024;25(8):3574-3600
EMBO Reports 2024;25(8):3574-3600
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and RASSF3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406009).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
- Sample type
- Human