PA5-84814

antibody from Invitrogen Antibodies

Targeting: CCK

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-84814

Provider

Invitrogen Antibodies

Product name

CCK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Immunogen sequence: LQRAEEAPRR QLRVSQRTDG ESRAHLGALL ARYIQQARKA PSGRMSIVKN LQNLDPSHRI SDRDYMG

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.10 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

AMOTL2‑knockdown promotes the proliferation, migration and invasion of glioma by regulating β‑catenin nuclear localization.

Chen X, Lu Y, Guo G, Zhang Y, Sun Y, Guo L, Li R, Nan Y, Yang X, Dong J, Jin X, Huang Q
Oncology reports 2021 Jul;46(1)

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Mechanism underlying long non‑coding RNA ILF3‑AS1‑mediated inhibition of cervical cancer cell proliferation, invasion and migration, and promotion of apoptosis.

Zhu L, Chen R, Jiang C, Xie Q, Zhao W, Gao X, Huang H
Molecular medicine reports 2021 Aug;24(2)

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CCK in human lymph node using CCK Polyclonal Antibody (Product # PA5-84814) shows no positivity in non-germinal center cells as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CCK in human liver using CCK Polyclonal Antibody (Product # PA5-84814) shows no positivity in hepatocytes as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CCK in human small intestine using CCK Polyclonal Antibody (Product # PA5-84814) shows strong cytoplasmic positivity in endocrine glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CCK in human duodenum using CCK Polyclonal Antibody (Product # PA5-84814) shows strong cytoplasmic positivity in endocrine glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CCK in human skeletal muscle using CCK Polyclonal Antibody (Product # PA5-84814) shows no positivity in myocytes as expected.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2. AMOTL2 inhibits the proliferation, migration and invasion of glioma cells. (A and B) The U87MG cell line was transfected with lentiviral vectors of AMOTL2 shRNA and overexpressed plasmids of AMOTL2. Reverse transcription-quantitative PCR and western blotting were performed to examine the AMOTL2 expression level with control and treatment groups. (C) Colony formation assays were conducted using the U87MG cell line to examine the effect of the expression of AMOTL2 in cell colony formation (n=3). (D) The effect of AMOTL2 on proliferation was examined by Cell Counting Kit-8 assay in U87MG and U251 cell lines (n=3). (E) The migrating ability of U87MG cells was detected by wound healing assay (n=5). (F) Transwell migration (upper images) and Matrigel (r) invasion (lower images) assays were conducted to investigate the effect of AMOTL2 knockdown on U87MG cell migration and invasion (n=5). Results are presented as the mean +- SD. *P