M-7773

MB antibody from Sigma-Aldrich

PVALB

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for M-7773

Antibody Data

Product number

M-7773

Provider

Sigma-Aldrich

Product name

Anti-MB

Provider product page

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 11

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Validation comment

Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.