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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- R32952 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Myoglobin Antibody (N-Terminal Region)
- Antibody type
- Polyclonal
- Description
- This highly specific Myoglobin antibody is suitable for use in Western blot/Immunohistochemistry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Myoglobin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HepG2, 2) HeLa, 3) HL-60 and 4) Jurkat cell lysate with Myoglobin antibody at 0.5ug/ml. Predicted molecular weight ~17 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HepG2, 2) HeLa, 3) HL-60 and 4) Jurkat cell lysate with Myoglobin antibody at 0.5ug/ml. Predicted molecular weight ~17 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat heart, 2) rat skeletal muscle, 3) mouse heart, 4) mouse skeletal muscle, 5) rat liver and 6) mouse liver tissue lysate with Myoglobin antibody at 0.5ug/ml. Predicted molecular weight ~17 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse skeletal muscle tissue with Myoglobin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat skeletal muscle tissue with Myoglobin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
