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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MAB9720-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Rat Myoglobin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Myoglobin in direct ELISAs. Detects human and rat Myoglobin in Western blots. In Western blots, no detection of mouse myglobin was observed.
- Reactivity
- Human, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P02144- Isotype
- IgG
- Antibody clone number
- 974102
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Rat Myoglobin by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue (negative control), rat heart tissue, and human liver tissue (negative control). PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human/Rat Myoglobin Monoclonal Antibody (Catalog # MAB9720) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Myoglobin at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Rat Myoglobin by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, rat heart tissue, and mouse heart tissue, loaded at 0.5 mg/mL. A specific band was detected for Myoglobin at approximately 22 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Rat Myoglobin Monoclonal Antibody (Catalog # MAB9720) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Myoglobin in Human Heart. Myoglobin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Rat Myoglobin Monoclonal Antibody (Catalog # MAB9720) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
