Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB97202-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Myoglobin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Myoglobin in direct ELISAs. In sandwich immunoassays, the antibody pair is specific for human Myoglobin.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P02144- Isotype
- IgG
- Antibody clone number
- 974145
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Myoglobin by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.5 mg/mL. A specific band was detected for Myoglobin at approximately 22 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Myoglobin Monoclonal Antibody (Catalog # MAB97202) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Myoglobin by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue (negative control), rat heart tissue (negative control), and human liver tissue (negative control). PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human Myoglobin Monoclonal Antibody (Catalog # MAB97202) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Myoglobin at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Myoglobin in Human Heart. Myoglobin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Myoglobin Monoclonal Antibody (Catalog # MAB97202) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
