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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-33110 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Myoglobin Monoclonal Antibody (7A3D2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7A3D2
- Vial size
- 100 µg
- Concentration
- 3.24 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Myoglobin using a Myoglobin Monoclonal antibody (Product # MA5-33110) at a concentration of 0.3 µg/mL. Lane 1: Mouse heart muscle lysate. Lane 2: Recombinant Myoglobin at 10µg. HRP labeled A secondary Goat polyclonal antibody to Mouse IgG was applied at a 1:3,000 dilution. Observed band size: 20 kDa. Additional bands at: 120 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Myoglobin using a Myoglobin Monoclonal antibody (Product # MA5-33110). Positive WB detected in: Mouse heart tissue, Mouse skeletal muscle tissue. All lanes: MB antibody at 1:2000. A secondary Goat polyclonal antibody to Mouse IgG was applied at a 1:50,000 dilution. Observed band size: 18 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Myoglobin using a Myoglobin Monoclonal antibody (Product # MA5-33110) at a concentration of 1 µg/mL. Lane 1: Myoglobin transfected 293 cell lysate. HRP labeled A secondary Goat polyclonal antibody to Mouse IgG was applied at a 1:3,000 dilution. Observed band size: 20 kDa. Additional bands at: 23, 25, 40 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Myoglobin in MCF-7 cells using a Myoglobin monoclonal antibody (Product # MA5-33110) at a dilution of 1:100. Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L) secondary antibody was used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Myoglobin in paraffin embedded human heart tissue using a Myoglobin monoclonal antibody (Product # MA5-33110) at a dilution of 1:100.
