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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-12299 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WIF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse, rat, Xenopus and zebrafish based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Perturbation of Wnt/β-catenin signaling and sexual dimorphism in non-alcoholic fatty liver disease.
Yeh MM, Shi X, Yang J, Li M, Fung KM, Daoud SS
Hepatology research : the official journal of the Japan Society of Hepatology 2022 May;52(5):433-448
Hepatology research : the official journal of the Japan Society of Hepatology 2022 May;52(5):433-448
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WIF1 (arrow) using a WIF1 polyclonal antibody (Product # PA5-12299) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the WIF1 gene (Lane 2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue using a WIF1 polyclonal antibody (Product # PA5-12299), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 4 FIGURE DEGs between male and female cases in NAFLD using Wnt focusing RT 2 profiler Polymerase chain reaction (PCR) arrays. (a) Scatter plot comparing the normalized average expression of each gene of the ""Wnt targets"" PCR array between the two groups (cases 8 & 5 ""NAFLD"" vs. cases 2 & 3 ""normal"" in female samples). The red and green dot stand for up-regulated and down-regulated genes, respectively, whereas black dots indicate unchanged genes; cut-off = +-1.5. (b) Visualization of log2 (Fold Change). Two groups of differential genes in female samples, intersect and merge gene expression colorimetry maps. Based on the expression levels of the DEGs in each in groups, a log2 is taken. Using systematic clustering (Hierarchical Cluster), the overall clustering result of the sample is obtained. Red indicates higher expression and blue indicates lower expression. (c) Scatter plot comparing the normalized average expression of each gene of the ""Wnt targets"" PCR array between the two groups (cases 6 & 7 ""NAFLD"" vs. cases 1 & 4 ""normal"") in male samples. (d) Visualization of log2 (Fold Change) of gene expression in male samples. (e) Protein expression of beta-catenin, FRZB, PITX2 and WIF1 in normal and NASH samples analyzed by immunoblotting. The samples include Normal 1 (male), Normal 2 (female), NASH 1 (male) and NASH 2 (female). Primary antibodies against beta-catenin, FRZB, PITX2 and WIF1 were used for detection. beta-Actin was used as the loading (reference) control.
