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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ASR-035-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 5HT2B Receptor (HTR2B) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse brain (lanes 1 and 5), rat brain (lanes 2 and 6), rat uterus (lanes 3 and 7) and human SH-SY5Y brain neuroblastoma cell (lanes 4 and 8) lysates: - 1-4. Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody (#ASR-035), (1:200).5-8. Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody , preincubated with 5HT2B Receptor/HTR2B (extracellular) Blocking Peptide (#BLP-SR035).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of 5-Hydroxytryptamine receptor 2B in mouse cerebellum - Immunohistochemical staining of mouse cerebellum using Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody (#ASR-035) (1:400). A. 5-HT2B staining (red), appears in neurons of the Purkinje layer (vertical arrows) and in the molecular layer (horizontal arrow). B. Nuclear staining using DAPI as the counterstaining (blue). C. Merged image of panels A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of 5-Hydroxytryptamine receptor 2B by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody (#ASR-035), (2.5μg) + goat- Anti-rabbit-FITC.
