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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-117130 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAB24 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: HT-1080, MCF7, SH-SY5Y, 293T, Mouse brain Immunogen sequence: MSGQRVDVKV VMLGKEYVGK TSLVERYVHD RFLVGPYQNT IGAAFVAKVM SVGDRTVTLG IWDTAGSERY EAMSRIYYRG AKAAIVCYDL TDSSSFERAK FWVKELRSLE EGCQIYLCGT KSDLLEEDRR RRRVDFHDVQ DYADNIKAQL FETSSKTGQS VDELFQKVAE DYVSVAAFQV MTEDKGVDLG QKPNPYFYSC CHH
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.68 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAB24 in extracts of various cell lines. Samples were incubated with RAB24 Polyclonal antibody (Product # PA5-117130) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of RAB24 in 200 μg extracts of MCF-7 cells. Samples were precipitated with 3 μg RAB24 Polyclonal antibody (Product # PA5-117130). Western blot was performed from the immunoprecipitate using RAB24 Polyclonal antibody (Product # PA5-117130) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of RAB24 in 200 µg extracts of MCF-7 cells using RAB24 Polyclonal Antibody (Product # PA5-117130). Western blot was performed from the immunoprecipitate using the same antibody at a dilution of 1:100
