18892-1-AP

antibody from Proteintech Group

Targeting: CAVIN1 cavin-1, CGL4, PTRF

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

18892-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#18892-1-AP, RRID:AB_10596795

Product name

PTRF antibody

Antibody type

Polyclonal

Description

KD/KO validated PTRF antibody (Cat. #18892-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Caveolin-1 protects endothelial cells from extensive expansion of transcellular tunnel by stiffening the plasma membrane.

Morel C, Lemerle E, Tsai FC, Obadia T, Srivastava N, Marechal M, Salles A, Albert M, Stefani C, Benito Y, Vandenesch F, Lamaze C, Vassilopoulos S, Piel M, Bassereau P, Gonzalez-Rodriguez D, Leduc C, Lemichez E
eLife 2024 Mar 22;12

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Early proteostasis of caveolins synchronizes trafficking, degradation, and oligomerization to prevent toxic aggregation.

Morales-Paytuví F, Fajardo A, Ruiz-Mirapeix C, Rae J, Tebar F, Bosch M, Enrich C, Collins BM, Parton RG, Pol A
The Journal of cell biology 2023 Sep 4;222(9)

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Inflammatory Cell-Derived MYDGF Attenuates Endothelial LDL Transcytosis to Protect Against Atherogenesis.

Xu J, Ma H, Shi L, Zhou H, Cheng Y, Tong J, Meng B, Xu X, He K, Ding S, Zhang J, Yue L, Xiang G
Arteriosclerosis, thrombosis, and vascular biology 2023 Nov;43(11):e443-e467

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Caveolae sense oxidative stress through membrane lipid peroxidation and cytosolic release of CAVIN1 to regulate NRF2.

Wu Y, Lim YW, Stroud DA, Martel N, Hall TE, Lo HP, Ferguson C, Ryan MT, McMahon KA, Parton RG
Developmental cell 2023 Mar 13;58(5):376-397.e4

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Serum amyloid A and interleukin -1β facilitate LDL transcytosis across endothelial cells and atherosclerosis via NF-κB/caveolin-1/cavin-1 pathway.

Jia X, Liu Z, Wang Y, Li G, Bai X
Atherosclerosis 2023 Jun;375:87-97

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PTRF/Cavin-1 enhances chemo-resistance and promotes temozolomide efflux through extracellular vesicles in glioblastoma.

Yang E, Wang L, Jin W, Liu X, Wang Q, Wu Y, Tan Y, Wang Y, Cui X, Zhao J, Tong F, Hong B, Xiao M, Liu X, Fang C, Kang C
Theranostics 2022;12(9):4330-4347

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DHCR24 Knockdown Induces Tau Hyperphosphorylation at Thr181, Ser199, Ser262, and Ser396 Sites via Activation of the Lipid Raft-Dependent Ras/MEK/ERK Signaling Pathway in C8D1A Astrocytes.

Mai M, Guo X, Huang Y, Zhang W, Xu Y, Zhang Y, Bai X, Wu J, Zu H
Molecular neurobiology 2022 Sep;59(9):5856-5873

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UBE2O ubiquitinates PTRF/CAVIN1 and inhibits the secretion of exosome-related PTRF/CAVIN1.

Cen X, Chen Q, Wang B, Xu H, Wang X, Ling Y, Zhang X, Qin D
Cell communication and signaling : CCS 2022 Nov 28;20(1):191

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A Novel High-Throughput Screening Method for a Human Multicentric Osteosarcoma-Specific Antibody and Biomarker Using a Phage Display-Derived Monoclonal Antibody.

Hayashi T, Yamamoto N, Kurosawa G, Tajima K, Kondo M, Hiramatsu N, Kato Y, Tanaka M, Yamaguchi H, Kurosawa Y, Yamada H, Fujita N
Cancers 2022 Nov 26;14(23)

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Blood exosomes-based targeted delivery of cPLA2 siRNA and metformin to modulate glioblastoma energy metabolism for tailoring personalized therapy.

Zhan Q, Yi K, Cui X, Li X, Yang S, Wang Q, Fang C, Tan Y, Li L, Xu C, Yuan X, Kang C
Neuro-oncology 2022 Nov 2;24(11):1871-1883

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Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis.

Andrade V, Bai J, Gupta-Rossi N, Jimenez AJ, Delevoye C, Lamaze C, Echard A
Science advances 2022 Apr 15;8(15):eabm5095

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PTRF/Cavin-1 as a Novel RNA-Binding Protein Expedites the NF-κB/PD-L1 Axis by Stabilizing lncRNA NEAT1, Contributing to Tumorigenesis and Immune Evasion in Glioblastoma.

Yi K, Cui X, Liu X, Wang Y, Zhao J, Yang S, Xu C, Yang E, Xiao M, Hong B, Fang C, Kang C, Tan Y, Wang Q
Frontiers in immunology 2021;12:802795

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Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.

Liao Z, Liu H, Ma L, Lei J, Tong B, Li G, Ke W, Wang K, Feng X, Hua W, Li S, Yang C
ACS nano 2021 Sep 28;15(9):14709-14724

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Salidroside-Mediated Autophagic Targeting of Active Src and Caveolin-1 Suppresses Low-Density Lipoprotein Transcytosis across Endothelial Cells.

Bai X, Jia X, Lu Y, Zhu L, Zhao Y, Cheng W, Shu M, Jin S
Oxidative medicine and cellular longevity 2020;2020:9595036

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Homotrimer cavin1 interacts with caveolin1 to facilitate tumor growth and activate microglia through extracellular vesicles in glioma.

Wang L, Yang C, Wang Q, Liu Q, Wang Y, Zhou J, Li Y, Tan Y, Kang C
Theranostics 2020;10(15):6674-6694

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EphB1 interaction with caveolin-1 in endothelial cells modulates caveolae biogenesis.

Tiruppathi C, Regmi SC, Wang DM, Mo GCH, Toth PT, Vogel SM, Stan RV, Henkemeyer M, Minshall RD, Rehman J, Malik AB
Molecular biology of the cell 2020 May 15;31(11):1167-1182

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A role for caveola-forming proteins caveolin-1 and CAVIN1 in the pro-invasive response of glioblastoma to osmotic and hydrostatic pressure.

Pu W, Qiu J, Nassar ZD, Shaw PN, McMahon KA, Ferguson C, Parton RG, Riggins GJ, Harris JM, Parat MO
Journal of cellular and molecular medicine 2020 Mar;24(6):3724-3738

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CAV1-CAVIN1-LC3B-mediated autophagy regulates high glucose-stimulated LDL transcytosis.

Bai X, Yang X, Jia X, Rong Y, Chen L, Zeng T, Deng X, Li W, Wu G, Wang L, Li Y, Zhang J, Xiong Z, Xiong L, Wang Y, Zhu L, Zhao Y, Jin S
Autophagy 2020 Jun;16(6):1111-1129

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Synaptotagmin-11 regulates the functions of caveolae and responds to mechanical stimuli in astrocytes.

Yan S, Wang Y, Zhang Y, Wang L, Zhao X, Du C, Gao P, Yan F, Liu F, Gong X, Guan Y, Cui X, Wang X, Xi Zhang C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Feb;34(2):2609-2624

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Correlation of the invasive potential of glioblastoma and expression of caveola-forming proteins caveolin-1 and CAVIN1.

Pu W, Nassar ZD, Khabbazi S, Xie N, McMahon KA, Parton RG, Riggins GJ, Harris JM, Parat MO
Journal of neuro-oncology 2019 Jun;143(2):207-220

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Cavin-1 regulates caveolae-mediated LDL transcytosis: crosstalk in an AMPK/eNOS/ NF-κB/Sp1 loop.

Bai XL, Yang XY, Li JY, Ye-Li, Jia X, Xiong ZF, Wang YM, Jin S
Oncotarget 2017 Nov 28;8(61):103985-103995

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Inducible Fgf13 ablation enhances caveolae-mediated cardioprotection during cardiac pressure overload.

Wei EQ, Sinden DS, Mao L, Zhang H, Wang C, Pitt GS
Proceedings of the National Academy of Sciences of the United States of America 2017 May 16;114(20):E4010-E4019

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Reactive oxygen species mediate angiotensin II-induced transcytosis of low-density lipoprotein across endothelial cells.

Bian F, Cui J, Zheng T, Jin S
International journal of molecular medicine 2017 Mar;39(3):629-635

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Deciphering caveolar functions by syndapin III KO-mediated impairment of caveolar invagination.

Seemann E, Sun M, Krueger S, Tröger J, Hou W, Haag N, Schüler S, Westermann M, Huebner CA, Romeike B, Kessels MM, Qualmann B
eLife 2017 Dec 5;6

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Acetylation of Cavin-1 Promotes Lipolysis in White Adipose Tissue.

Zhou SR, Guo L, Wang X, Liu Y, Peng WQ, Liu Y, Wei XB, Dou X, Ding M, Lei QY, Qian SW, Li X, Tang QQ
Molecular and cellular biology 2017 Aug 15;37(16)

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Src-dependent phosphorylation of caveolin-1 Tyr-14 promotes swelling and release of caveolae.

Zimnicka AM, Husain YS, Shajahan AN, Sverdlov M, Chaga O, Chen Z, Toth PT, Klomp J, Karginov AV, Tiruppathi C, Malik AB, Minshall RD
Molecular biology of the cell 2016 Jul 1;27(13):2090-106

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Non-caveolar caveolin-1 expression in prostate cancer cells promotes lymphangiogenesis.

Nassar ZD, Hill MM, Parton RG, Francois M, Parat MO
Oncoscience 2015;2(7):635-45

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Hypoxia inhibits Cavin-1 and Cavin-2 expression and down-regulates caveolae in adipocytes.

Regazzetti C, Dumas K, Lacas-Gervais S, Pastor F, Peraldi P, Bonnafous S, Dugail I, Le Lay S, Valet P, Le Marchand-Brustel Y, Tran A, Gual P, Tanti JF, Cormont M, Giorgetti-Peraldi S
Endocrinology 2015 Mar;156(3):789-801

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Cavin-1/PTRF alters prostate cancer cell-derived extracellular vesicle content and internalization to attenuate extracellular vesicle-mediated osteoclastogenesis and osteoblast proliferation.

Inder KL, Ruelcke JE, Petelin L, Moon H, Choi E, Rae J, Blumenthal A, Hutmacher D, Saunders NA, Stow JL, Parton RG, Hill MM
Journal of extracellular vesicles 2014;3

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Mitsugumin 53 protects the kidney from severe burn injury in mice.

Wu Y, Huang J, Liu D, Tan J, Peng Y, Yang J, Cui Y, He W, Luo G, Wu J
Burns & trauma 2013;1(3):128-33

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PTRF/Cavin-1 decreases prostate cancer angiogenesis and lymphangiogenesis.

Nassar ZD, Moon H, Duong T, Neo L, Hill MM, Francois M, Parton RG, Parat MO
Oncotarget 2013 Oct;4(10):1844-55

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 18892-1-AP(PTRF antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using PTRF antibody 18892-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-PTRF (IP:18892-1-AP, 3ug; Detection:18892-1-AP 1:800) with mouse lung tissue lysate 5160ug.

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human pancreas using 18892-1-AP(PTRF antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue