12055-2-AP

antibody from Proteintech Group

Targeting: RTN3 ASYIP, HAP, NSPL2, NSPLII, RTN3-A1

Provider product page for 12055-2-AP

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [41]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 12055-2-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12055-2-AP, RRID:AB_2301357
Product name: RTN3 antibody
Antibody type: Polyclonal
Description: RTN3 antibody (Cat. #12055-2-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IHC, IP, WB,ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

RTN3 protein structure - 12055-2-AP shown in red.

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The Journal of biological chemistry 2026 Mar;302(3):111147

Reticulon 3 deficiency induces ferroptosis via chaperone-mediated autophagy in ischemia-reperfusion induced acute kidney injury.

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Ageing promotes microglial accumulation of slow-degrading synaptic proteins.

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Nature 2026 Feb;650(8103):930-941

Autophagy regulates PVALB (parvalbumin) interneuron excitability and memory.

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Autophagy 2026 Feb;22(2):351-370

Citrulline Modulates Milk Fat Metabolism via the RTN3-FABP5 Axis in Bovine Mammary Epithelial Cells.

Cheng J, Ren K, Shi Y, Wang L, Li L, Yang Y, Kuang T, Pan S
Journal of agricultural and food chemistry 2026 Apr 8;74(13):11079-11091

Poly(ADP-ribose) Polymerase 1 Deficiency Attenuates Amyloid Pathology, Neurodegeneration, and Cognitive Decline in a Familial Alzheimer's Disease Model.

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Integrative Mechanistic Studies Identify Reticulon-3 as a Critical Modulator of Infectious Exosome-Driven Dengue Pathogenesis.

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The EMBO journal 2025 Feb;44(4):1039-1073

RETREG1-mediated reticulophagy is activated by an ATF4-CEBPG/C/EBPγ heterodimer and confers protection against lipotoxicity.

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AGPAT2 acts at the crossroads of lipid biosynthesis and DRP1-mediated ER morphogenesis.

Adachi Y, Torres M, Hunter AH, Cho WJ, Pan M, Han X, Du G, Qi L, Iijima M, Sesaki H
Nature communications 2025 Dec 12;16(1):11473

Physical exercise mitigates the cognitive impairments by promoting ER-phagy in mice model of Alzheimer's disease.

Li L, Li S, Cai L, Yang X, Feng Y, Zhang L, He X, Li M
Behavioural brain research 2025 Aug 24;492:115650

A multi-subunit autophagic capture complex facilitates degradation of ER stalled MHC-I in pancreatic cancer.

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ER-phagy restrains inflammatory responses through its receptor UBAC2.

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AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity.

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Drp1 Aggravates Copper Nanoparticle-Induced ER-Phagy by Disturbing Mitochondria-Associated Membranes in Chicken Hepatocytes.

Li Q, Guo P, Wang S, Su L, Yu W, Guo J, Hu L, Zhang H, Pan J, Tang Z, Liao J
Journal of agricultural and food chemistry 2024 Jul 24;72(29):16506-16518

Endoplasmic reticulum stress of endometrial mesenchymal stem cells in endometriosis.

Rencber SF, Yazır Y, Sarıhan M, Sezer Z, Korun ZEU, Ozturk A, Duruksu G, Guzel E, Akpınar G, Corakci A
Tissue & cell 2024 Dec;91:102544

Ubiquitin Ligase ITCH Regulates Life Cycle of SARS-CoV-2 Virus.

Xiang Q, Wouters C, Chang P, Lu YN, Liu M, Wang H, Yang J, Pekosz A, Zhang Y, Wang J
bioRxiv : the preprint server for biology 2024 Dec 5;

Fam134c and Fam134b shape axonal endoplasmic reticulum architecture in vivo.

Iavarone F, Zaninello M, Perrone M, Monaco M, Barth E, Gaedke F, Pizzo MT, Di Lorenzo G, Desiderio V, Sommella E, Merciai F, Salviati E, Campiglia P, Luongo L, De Leonibus E, Rugarli E, Settembre C
EMBO reports 2024 Aug;25(8):3651-3677

Downregulating miR-432-5p exacerbates adriamycin-induced cardiotoxicity via activating the RTN3 signaling pathway.

Geng W, Yan S, Sang D, Tao J, Zhang X, Gu X, Zhang X
Aging 2024 Aug 22;16(16):11904-11916

Integrated proteomics reveals autophagy landscape and an autophagy receptor controlling PKA-RI complex homeostasis in neurons.

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Nature communications 2024 Apr 10;15(1):3113

Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice.

Huang HZ, Ai WQ, Wei N, Zhu LS, Liu ZQ, Zhou CW, Deng MF, Zhang WT, Zhang JC, Yang CQ, Hu YZ, Han ZT, Zhang HH, Jia JJ, Wang J, Liu FF, Li K, Xu Q, Yuan M, Man H, Guo Z, Lu Y, Shu K, Zhu LQ, Liu D
Protein & cell 2024 Apr 1;15(4):261-284

Integrative proteomics highlight presynaptic alterations and c-Jun misactivation as convergent pathomechanisms in ALS.

Aly A, Laszlo ZI, Rajkumar S, Demir T, Hindley N, Lamont DJ, Lehmann J, Seidel M, Sommer D, Franz-Wachtel M, Barletta F, Heumos S, Czemmel S, Kabashi E, Ludolph A, Boeckers TM, Henstridge CM, Catanese A
Acta neuropathologica 2023 Sep;146(3):451-475

Adaptive responses of neuronal cells to chronic endoplasmic reticulum (ER) stress.

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Redox biology 2023 Nov;67:102943

Melatonin restores endoplasmic reticulum homeostasis to protect injured neurons in a rat model of chronic cervical cord compression.

Yao M, Pu PM, Li ZY, Zhu K, Zhou LY, Sun YL, Dai YX, Cui XJ, Wang YJ
Journal of pineal research 2023 May;74(4):e12859

Deficiency of Perry syndrome-associated p150(Glued) in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities.

Yu J, Yang X, Zheng J, Sgobio C, Sun L, Cai H
NPJ Parkinson's disease 2023 Mar 7;9(1):35

Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy.

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Fibroblast exosomal TFAP2C induced by chitosan oligosaccharides promotes peripheral axon regeneration via the miR-132-5p/CAMKK1 axis.

Zhao Y, Liu J, Liu S, Yang P, Liang Y, Ma J, Mao S, Sun C, Yang Y
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CCPG1 recognizes endoplasmic reticulum luminal proteins for selective ER-phagy.

Ishii S, Chino H, Ode KL, Kurikawa Y, Ueda HR, Matsuura A, Mizushima N, Itakura E
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Liver X receptor-agonist treatment rescues degeneration in a Drosophila model of hereditary spastic paraplegia.

Byrne DJ, Garcia-Pardo ME, Cole NB, Batnasan B, Heneghan S, Sohail A, Blackstone C, O'Sullivan NC
Acta neuropathologica communications 2022 Mar 28;10(1):40

ER proteins decipher the tubulin code to regulate organelle distribution.

Zheng P, Obara CJ, Szczesna E, Nixon-Abell J, Mahalingan KK, Roll-Mecak A, Lippincott-Schwartz J, Blackstone C
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Franjic D, Skarica M, Ma S, Arellano JI, Tebbenkamp ATN, Choi J, Xu C, Li Q, Morozov YM, Andrijevic D, Vrselja Z, Spajic A, Santpere G, Li M, Zhang S, Liu Y, Spurrier J, Zhang L, Gudelj I, Rapan L, Takahashi H, Huttner A, Fan R, Strittmatter SM, Sousa AMM, Rakic P, Sestan N
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Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.

Zhu PP, Hung HF, Batchenkova N, Nixon-Abell J, Henderson J, Zheng P, Renvoisé B, Pang S, Xu CS, Saalfeld S, Funke J, Xie Y, Svara F, Hess HF, Blackstone C
Human molecular genetics 2022 Aug 23;31(16):2779-2795

ATF4 links ER stress with reticulophagy in glioblastoma cells.

Zielke S, Kardo S, Zein L, Mari M, Covarrubias-Pinto A, Kinzler MN, Meyer N, Stolz A, Fulda S, Reggiori F, Kögel D, van Wijk S
Autophagy 2021 Sep;17(9):2432-2448

TAOK2 is an ER-localized kinase that catalyzes the dynamic tethering of ER to microtubules.

Nourbakhsh K, Ferreccio AA, Bernard MJ, Yadav S
Developmental cell 2021 Dec 20;56(24):3321-3333.e5

Quantitative Proteomics and Differential Protein Abundance Analysis after the Depletion of PEX3 from Human Cells Identifies Additional Aspects of Protein Targeting to the ER.

Zimmermann R, Lang S, Lerner M, Förster F, Nguyen D, Helms V, Schrul B
International journal of molecular sciences 2021 Dec 1;22(23)

Knockdown of Long Non-Coding RNA AFAP1-AS1 Promoted Viability and Suppressed Death of Cardiomyocytes in Response to I/R In Vitro and In Vivo.

Chen Z, Wang X, Hou X, Ding F, Yi K, Zhang P, You T
Journal of cardiovascular translational research 2020 Dec;13(6):996-1007

TMCC3 localizes at the three-way junctions for the proper tubular network of the endoplasmic reticulum.

Wisesa S, Yamamoto Y, Sakisaka T
The Biochemical journal 2019 Nov 15;476(21):3241-3260

ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.

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Intrinsically Disordered Protein TEX264 Mediates ER-phagy.

Chino H, Hatta T, Natsume T, Mizushima N
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The peroxisome biogenesis factors posttranslationally target reticulon homology domain-containing proteins to the endoplasmic reticulum membrane.

Yamamoto Y, Sakisaka T
Scientific reports 2018 Feb 2;8(1):2322

Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.

Wang T, Wang B, Huang H, Zhang C, Zhu Y, Pei B, Cheng C, Sun L, Wang J, Jin Q, Zhao Z
PLoS pathogens 2017 Oct;13(10):e1006674

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 12055-2-AP(RTN3 antibody) at dilution of 1:400
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human gliomas using 12055-2-AP(RTN3 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The RTN3 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human RTN3. This antibody recognizes human, mouse, rat antigen. The RTN3 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 12055-2-AP.pdf

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