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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-78316 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RTN3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: HeLa, HeLa membrane fraction extract Predicted Reactivity: Chimpanzee (98%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.69 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RTN3 in HeLa cells using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with RTN3 polyclonal antibody (Product # PA5-78316) using a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RTN3 Polyclonal Antibody detects RTN3 protein by western blot analysis. HeLa whole cell extracts and membrane extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with RTN3 Polyclonal Antibody (Product # PA5-78316) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of RTN3 protein from HeLa whole cell extracts using 5 µg of RTN3 Polyclonal Antibody (Product # PA5-78316). Western blot analysis was performed using RTN3 Polyclonal Antibody (Product # PA5-78316) diluted at 1:500.
