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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- A302-860A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Reticulon-3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Reticulon-3 by western blot. Samples: Whole cell lysate (5, 15, and 50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-Reticulon-3 antibody (Product # A302-860A; lot A302-860A-1) used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Reticulon-3 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Reticulon-3 antibody A302-860A used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. Reticulon-3 was also immunoprecipitated by rabbit anti-Reticulon-3 antibody A302-859A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Reticulon-3 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- Reticulon-3 (Cat. No. A302-860A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Reticulon-3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Reticulon-3 antibody (Product # A302-860A; lot A302-860A-1) used for IP at 3 µg/mg lysate. Reticulon-3 was also immunoprecipitated by rabbit anti-Reticulon-3 antibody (Product # A302-859A), which recognizes an upstream epitope. For blotting immunoprecipitated Reticulon-3 (Product # A302-860A) was used at 0.4 µg/mL. Detection: Chemiluminescence with exposure time of 3 seconds.
