MA5-15538

antibody from Invitrogen Antibodies

Targeting: RTN3 ASYIP, HAP, NSPL2, NSPLII, RTN3-A1

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15538

Provider

Invitrogen Antibodies

Product name

RTN3 Monoclonal Antibody (1E11)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15538 targets RTN3 in indirect ELISA, IF applications and shows reactivity with Human samples. The MA5-15538 immunogen is purified recombinant fragment of RTN3 expressed in E. Coli. MA5-15538 detects RTN3 which has a predicted molecular weight of approximately 113kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1E11

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Rab32 uses its effector reticulon 3L to trigger autophagic degradation of mitochondria-associated membrane (MAM) proteins.

Herrera-Cruz MS, Yap MC, Tahbaz N, Phillips K, Thomas L, Thomas G, Simmen T
Biology direct 2021 Nov 7;16(1):22

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa (A), A431 (B) and THP-1 (C) cells using RTN3 monoclonal antibody (Product # MA5-15538) (Green). Blue: DRAQ5 fluorescent DNA dye.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3 Rab32 interacts with the ER-phagy receptor Reticulon-3. A Co-immunoprecipitation of Rab32 constructs with endogenous RTN3L. MCF7 cells were transfected for 48 h with FLAG-tagged Rab32 constructs, followed by crosslinking, lysis and incubation with anti-FLAG antibodies. Immunoprecipitates were analyzed for anti-FLAG and co-immunoprecipitating endogenous long reticulon-3 (RTN3L). Densitometry of RTN3L binding normalized to respective input signals. n = 3, * p 0.05. C Co-immunoprecipitation of endogenous Rab32 with HA-tagged RTN3L. MCF7 cells were transfected with HA-tagged RTN3L, followed by lysis and incubation with anti-HA antibodies. Immunoprecipitates were analyzed for anti-HA and co-immunoprecipitating endogenous Rab32. D Co-immunoprecipitation of FLAG-Rab32 constructs expressed from pcDNA3 with HA-tagged RTN3L and deletion mutants, expressed from pCEP4. HeLa cells were transfected for 48 h with FLAG-tagged Rab32 and HA-tagged RTN3L constructs, followed by crosslinking, lysis and incubation with anti-HA antibodies. Immunoprecipitates were analyzed

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 Rab32 cooperates with the RTN3 long form. A Immunoblot and densitometry analysis of control pcDNA3 and Q85L-FLAG transfected cells indicates only RTN3L levels decrease with Q85L, while RTN3S, FAM134B, Sec62, and CCPG1 do not show changes. n = 3; ** p