MA5-15538

antibody from Invitrogen Antibodies

Targeting: RTN3 ASYIP, HAP, NSPL2, NSPLII, RTN3-A1

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15538

Provider

Invitrogen Antibodies

Product name

RTN3 Monoclonal Antibody (1E11)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15538 targets RTN3 in IF applications and shows reactivity with Human samples.

Antibody clone number

1E11

Concentration

Conc. Not Determined

References

Submitted references

Rab32 uses its effector reticulon 3L to trigger autophagic degradation of mitochondria-associated membrane (MAM) proteins.

Herrera-Cruz MS, Yap MC, Tahbaz N, Phillips K, Thomas L, Thomas G, Simmen T
Biology direct 2021 Nov 7;16(1):22

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa (A), A431 (B) and THP-1 (C) cells using RTN3 monoclonal antibody (Product # MA5-15538) (Green). Blue: DRAQ5 fluorescent DNA dye.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3 Rab32 interacts with the ER-phagy receptor Reticulon-3. A Co-immunoprecipitation of Rab32 constructs with endogenous RTN3L. MCF7 cells were transfected for 48 h with FLAG-tagged Rab32 constructs, followed by crosslinking, lysis and incubation with anti-FLAG antibodies. Immunoprecipitates were analyzed for anti-FLAG and co-immunoprecipitating endogenous long reticulon-3 (RTN3L). Densitometry of RTN3L binding normalized to respective input signals. n = 3, * p 0.05. C Co-immunoprecipitation of endogenous Rab32 with HA-tagged RTN3L. MCF7 cells were transfected with HA-tagged RTN3L, followed by lysis and incubation with anti-HA antibodies. Immunoprecipitates were analyzed for anti-HA and co-immunoprecipitating endogenous Rab32. D Co-immunoprecipitation of FLAG-Rab32 constructs expressed from pcDNA3 with HA-tagged RTN3L and deletion mutants, expressed from pCEP4. HeLa cells were transfected for 48 h with FLAG-tagged Rab32 and HA-tagged RTN3L constructs, followed by crosslinking, lysis and incubation with anti-HA antibodies. Immunoprecipitates were analyzed

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 Rab32 cooperates with the RTN3 long form. A Immunoblot and densitometry analysis of control pcDNA3 and Q85L-FLAG transfected cells indicates only RTN3L levels decrease with Q85L, while RTN3S, FAM134B, Sec62, and CCPG1 do not show changes. n = 3; ** p