Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-22966 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-22966, RRID:AB_1726518
- Product name
- Rabbit Polyclonal DDIT4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Influenza virus differentially activates mTORC1 and mTORC2 signaling to maximize late stage replication.
Mode of action and pharmacogenomic biomarkers for exceptional responders to didemnin B.
Kuss-Duerkop SK, Wang J, Mena I, White K, Metreveli G, Sakthivel R, Mata MA, Muñoz-Moreno R, Chen X, Krammer F, Diamond MS, Chen ZJ, García-Sastre A, Fontoura BMA
PLoS pathogens 2017 Sep;13(9):e1006635
PLoS pathogens 2017 Sep;13(9):e1006635
Mode of action and pharmacogenomic biomarkers for exceptional responders to didemnin B.
Potts MB, McMillan EA, Rosales TI, Kim HS, Ou YH, Toombs JE, Brekken RA, Minden MD, MacMillan JB, White MA
Nature chemical biology 2015 Jun;11(6):401-8
Nature chemical biology 2015 Jun;11(6):401-8
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDIT4 Antibody [NBP1-22966] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Lysate was prepared from cells that had been treated with cobalt chloride (A and B) or mock treated (A). NBP1-22966 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDIT4 Antibody [NBP1-22966] - Influenza virus down-regulates REDD1 to promote mTORC1 activity. A549 cells were infected with WSN at MOI of 2 PFU/cell for the indicated times. A549 cells were transfected with siRNAs (pool of three each) targeting viral mRNAs and then infected for 7 h at MOI of 2 PFU/cell. Immunoblot analysis was performed to detect the depicted proteins, n = 3. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.ppat.1006635), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDIT4 Antibody [NBP1-22966] - Influenza virus down-regulates REDD1 to promote mTORC1 activity.(A-C) A549 cells were infected with WSN at MOI of 2 PFU/cell for the indicated times. A549 cells were transfected with siRNAs (pool of three each) targeting viral mRNAs and then infected for 7 h at MOI of 2 PFU/cell. Immunoblot analysis was performed to detect the depicted proteins, n = 3. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.ppat.1006635), licensed under a CC-BY licence.
