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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-38201 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-OR13D1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Gegen Qinlian Decoction Coordinately Regulates PPARĪ³ and PPARĪ± to Improve Glucose and Lipid Homeostasis in Diabetic Rats and Insulin Resistance 3T3-L1 Adipocytes.
Tu J, Zhu S, Li B, Xu G, Luo X, Jiang L, Yan X, Zhang R, Chen C
Frontiers in pharmacology 2020;11:811
Frontiers in pharmacology 2020;11:811
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of OR13D1 in extracts from RAW264.7 cells using an OR13D1 polyclonal antibody (Product # PA5-38201).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 9 The effect of serum (GQD-CS) with PPARgamma/PPARalpha activation in the IR-3T3-L1 adipocytes. (A) PPARalpha/PPARgamma protein binding activities with target peroxisome proliferator response element (PPRE) element, n=5 per group. (B) Total PPARalpha/PPARgamma protein levels and nuclear PPARalpha/PPARgamma protein levels. (C) PPARgamma/alpha upstream phosphorylated kinase expression including p-p38MAPK, p-ERK1/2, CDK5, and deacetylase SIRT1 expression. (D) PPARgamma/alpha downstream target protein. (E) Other nuclear transcription factors crosstalk with PPARgamma/alpha. In all WB experiments, data showed representing per group of n = 3, all values are expressed as the mean +- SEM. * P < 0.05, ** P < 0.01, and *** P < 0.001 when compared with IR-3T3-L1 group. The target protein expression levels were normalized to that of beta-actin or beta-tubulin expression levels. The nuclear protein expression levels were normalized to that of Lamin B expression level.
