Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- 16258-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RGS14 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: KSLPLGVEE LGQLPPVEGP GGRPLRKSFR RELGGTANAA LRRESQGSLN SSASLDLGFL AFVSSKSESH RKSLGSTEGE SESRPGKYCC VYLPDGTASL ALARPGLTIR DMLAGICEKR GLSLPDIKVY LVGNEQALVL DQDCTVLADQ EVRLENRITF ELELTALERV VRISAKPTKR LQEALQPILE KHGLSPLEVV LHRPGEKQPL DLGKLVSSVA AQRLVLDTLP GVKISKARDK SPCRSQGCPP RTQDKATHPP PASPSSLVKV PSSATGKRQT CDIEGLVELL NRVQSSGAHD QRGLLRKEDL VLPEFLQLPA QGPSSEETPP QTKSAAQPIG GSLNSTTDSA L (217-566 aa encoded by BC014094)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16258-1-AP (RGS14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 16258-1-AP (RGS14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 16258-1-AP (RGS14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 16258-1-AP (RGS14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 16258-1-AP (RGS14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 16258-1-AP (RGS14 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 16258-1-AP ( RGS14 Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 16258-1-AP ( RGS14 Antibody) at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using (Product # 16258-1-AP) (RGS14 antibody) at dilution of 1:5,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RGS14 (IP:16258-1-AP, 3ug; Detection:16258-1-AP 1:1000) with mouse brain tissue lysate 5000ug.