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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-27174 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FZD10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: H1299, NIH-3T3, JC, BCL-1. Predicted reactivity: Mouse (100%), Rat (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Frizzled 10 using 30 µg of A) NIH-3T3 (B) JC and C) BCL-1 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Frizzled 10 polyclonal antibody (Product # PA5-27174) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Frizzled 10 using 30 µg of H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Frizzled 10 polyclonal antibody (Product # PA5-27174) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FZD10 Polyclonal Antibody (Product # PA5-27174). Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FZD10 Polyclonal Antibody (Product # PA5-27174) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FZD10 Polyclonal Antibody detects Frizzled 10 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Frizzled 10 stained by FZD10 Polyclonal Antibody (Product # PA5-27174) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
