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- Antibody Data
- Antigen structure
- References [1]
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- Product number
- PA5-25392 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DYNC1LI2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with non-human primate based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Dynein Light Intermediate Chain 2 Facilitates the Metaphase to Anaphase Transition by Inactivating the Spindle Assembly Checkpoint.
Mahale SP, Sharma A, Mylavarapu SV
PloS one 2016;11(7):e0159646
PloS one 2016;11(7):e0159646
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 2 LIC2 is required for metaphase to anaphase progression. A) Metaphase index (% of total cells present in metaphase +/- SD) in Hela cells treated with respective siRNAs as indicated. (3 experiments, n = approximately 500 cells per experiment). B) & C) Western blots showing siRNA mediated LIC1 and LIC2 specific knockdown. LIC2a and LIC2b represent two different siRNA sequences against human LIC2 [ 23 ]. The band marked by the arrow represents human LIC2 that gets reduced upon siRNA treatment, the upper bands are non-specifically recognized by the LIC2 antibody (ThermoScientific). Actin = loading control. D) Rescue of the LIC2-depletion induced metaphase arrest by transgenic expression of rat LIC2 (3 experiments, n = at least 500 cells per experiment). E) Western blots showing specific depletion of LIC2 upon treatment with LIC2-specific siRNAs, using a different LIC2 antibody (Abcam). LIC2a and LIC2b represent two different siRNA sequences against human LIC2 [ 23 ]. The band marked by the arrow represents human LIC2 that appears at the same molecular weight as in C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 7 LIC2 biochemically interacts with key SAC proteins at metaphase. A) Western blots of affinity purified mitotic LIC2 (LIC2-MTAP) probing for interaction of SAC proteins Mad1, Zw10 and BubR1 (right panel), and with the empty tag control (left panel). ss-actin was used as the loading control. Control indicates empty tag (MTAP vector alone) pulldown, LIC2 indicates LIC2-MTAP (see Materials and Methods ). B) Upper panels: Immunoblots probing for the pulldown of dynein subunits DHC (dynein heavy chain) and IC-74 (dynein intermediate chain) upon affinity purification of the empty MTAP tag or LIC2-MTAP. Lower panels show successful pulldown of the respective bait proteins as positive controls for the affinity purification reaction. IN = input, FT = flow through, WS = wash, AP = affinity pulldown precipitate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 1 Localization of LICs in Mitosis. A) Confocal immunofluorescence images of Hela cells depicting the localization of LIC1 (red) and LIC2 (green), shown by arrows. B) Fluorescence images of Hela cells showing the presence of LIC2 (green, using the ThermoScientific antibody) at kinetochores (CREST, red) in prometaphase. C) Fluorescence imaging after treatment of cells with anti-LIC2 siRNA, showing loss of LIC2 signal from spindle poles and kinetochores (arrow in inset). DAPI was used to visualize chromosomes. Scalebar is 5 mum in all images that are not zoomed.
