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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- H00116154-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00116154-M02, RRID:AB_1137359
- Product name
- PHACTR3 monoclonal antibody (M02), clone 4A5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant PHACTR3.
- Antigen sequence
TSALEKKMAGRQGREELIKKGLLEMMEQDAESKTC
NPDGGPRSVQSEPPTPKSETLTSEDAQPGSPLATG
TDQVSLDKPLSSAAHLDD- Isotype
- IgG
- Antibody clone number
- 4A5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PHACTR3 expression in transfected 293T cell line by PHACTR3 monoclonal antibody (M02), clone 4A5.Lane 1: PHACTR3 transfected lysate(51.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PHACTR3 is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
