PA5-88272

antibody from Invitrogen Antibodies

Targeting: PDGFB SIS, SSV

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-88272

Provider

Invitrogen Antibodies

Product name

PDGF-BB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Samples: SW480, MCF7, Mouse skeletal muscle Immunogen sequence: QCRPTQVQLR PVQVRKIEIV RKKPIFKKAT VTLEDHLACK CETVAAARPV TRSPGGSQEQ RAKTPQTRVT IRTVRVRRPP KGKHRKFKHT HDKTALKETL GA

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.58 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

Platelet-Derived Growth Factor-BB Inhibits Intervertebral Disc Degeneration via Suppressing Pyroptosis and Activating the MAPK Signaling Pathway.

Zhang W, Gong Y, Zheng X, Qiu J, Jiang T, Chen L, Lu F, Wu X, Cheng F, Hong Z
Frontiers in pharmacology 2021;12:799130

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PDGF-BB in extracts of various cell lines. Samples were incubated with PDGF-BB Polyclonal antibody (Product # PA5-88272) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PDGF-BB in HeLa cells. Samples were incubated with PDGF-BB Polyclonal antibody (Product # PA5-88272) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PDGF-BB in paraffin-embedded human placenta. Samples were incubated with PDGF-BB Polyclonal antibody (Product # PA5-88272) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PDGF-BB in paraffin-embedded rat heart. Samples were incubated with PDGF-BB Polyclonal antibody (Product # PA5-88272) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PDGF-BB in paraffin-embedded human placenta. Samples were incubated with PDGF-BB Polyclonal antibody (Product # PA5-88272) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.